中国科学院长春光学精密机械与物理研究所

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	Single-Cell Analysis and Classification according to Multiplexed Proteins via Microdroplet-Based Self-Driven Magnetic Surface-Enhanced Raman Spectroscopy Platforms Assisted with Machine Learning Algorithms
	J. Wang, L. Cong, W. Shi, W. Xu and S. Xu
	2023
发表期刊	Analytical Chemistry
ISSN	00032700
卷号	95期号:29页码:11019-11027
摘要	A microdroplet-based surface-enhanced Raman spectroscopy (microdroplet SERS) platform was constructed to envelop individual cells in microdroplets, followed by the SERS detection of their extracellular vesicle-proteins (EV-proteins) via the in-drop immunoassays by use of immunomagnetic beads (iMBs) and immuno-SERS tags (iSERS tags). A unique phenomenon is found that iMBs can start a spontaneous reorientation on the probed cell surface based on the electrostatic force-driven interfacial aggregation effect, which leads EV-proteins and iSERS tags to be gathered from a liquid phase to a cell membrane interface and significantly improves SERS sensitivity to the single-cell analysis level due to the formation of numbers of SERS hotspots. Three EV-proteins from two breast cancer cell lines were collected and further analyzed by machine learning algorithmic tools, which will be helpful for a deeper understanding of breast cancer subtypes from the view of EV-proteins. © 2023 American Chemical Society.
DOI	10.1021/acs.analchem.3c01273
URL	查看原文
收录类别	sci ; ei
引用统计
文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/67934
专题	中国科学院长春光学精密机械与物理研究所
推荐引用方式 GB/T 7714	J. Wang, L. Cong, W. Shi, W. Xu and S. Xu. Single-Cell Analysis and Classification according to Multiplexed Proteins via Microdroplet-Based Self-Driven Magnetic Surface-Enhanced Raman Spectroscopy Platforms Assisted with Machine Learning Algorithms[J]. Analytical Chemistry,2023,95(29):11019-11027.
APA	J. Wang, L. Cong, W. Shi, W. Xu and S. Xu.(2023).Single-Cell Analysis and Classification according to Multiplexed Proteins via Microdroplet-Based Self-Driven Magnetic Surface-Enhanced Raman Spectroscopy Platforms Assisted with Machine Learning Algorithms.Analytical Chemistry,95(29),11019-11027.
MLA	J. Wang, L. Cong, W. Shi, W. Xu and S. Xu."Single-Cell Analysis and Classification according to Multiplexed Proteins via Microdroplet-Based Self-Driven Magnetic Surface-Enhanced Raman Spectroscopy Platforms Assisted with Machine Learning Algorithms".Analytical Chemistry 95.29(2023):11019-11027.

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