A handheld continuous-flow real-time fluorescence qPCR system with a PVC microreactor

doi:10.1039/c9an01894h

CIOMP OpenIR

	A handheld continuous-flow real-time fluorescence qPCR system with a PVC microreactor
	B. Shi,Y. M. Li,D. Wu and W. M. Wu
	2020
发表期刊	Analyst
ISSN	0003-2654
卷号	145 期号:7 页码:2767-2773
摘要	The polymerase chain reaction (PCR) has unique advantages of sensitivity, specificity and rapidity in pathogen detection, which makes it at the forefront of academia and application in molecular biology diagnosis. In this study, we proposed a hand-held real-time fluorescence qPCR system, which can be used for the quantitative analysis of nucleic acid molecules. For the first time, we use a PVC microreactor which improved the transmittance of the microreactor and made it easy to collect the fluorescence signal. In order to make it portable, the system adopted a passive syringe for sample injection and integrated temperature control and detection with a lithium battery for power supply. What's more, the fluorescence signal was captured by using a smartphone through an external automatic robotic arm. This real-time qPCR system can detect genomic DNA of the H7N9 avian influenza over four orders of magnitude of concentration from 10(7) to 10(4) copies per mu L. In addition, it was verified that the fluorescence images obtained by this system were clearer than those obtained by a traditional system (using a PTFE spatial PCR microreactor) with two typical dyes and a probe tested-EvaGreen, SYBR Green and FAM.
DOI	10.1039/c9an01894h
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收录类别	SCI ; EI
语种	英语
引用统计
文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/64548
专题	中国科学院长春光学精密机械与物理研究所
推荐引用方式 GB/T 7714	B. Shi,Y. M. Li,D. Wu and W. M. Wu. A handheld continuous-flow real-time fluorescence qPCR system with a PVC microreactor[J]. Analyst,2020,145(7):2767-2773.
APA	B. Shi,Y. M. Li,D. Wu and W. M. Wu.(2020).A handheld continuous-flow real-time fluorescence qPCR system with a PVC microreactor.Analyst,145(7),2767-2773.
MLA	B. Shi,Y. M. Li,D. Wu and W. M. Wu."A handheld continuous-flow real-time fluorescence qPCR system with a PVC microreactor".Analyst 145.7(2020):2767-2773.

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