A new EV71 VP3 epitope in norovirus P particle vector displays neutralizing activity and protection in vivo in mice

	A new EV71 VP3 epitope in norovirus P particle vector displays neutralizing activity and protection in vivo in mice
	Jiang, L. P.; R. J. Fan; S. Y. Sun; P. Fan; W. H. Su; Y. Zhou; F. Gao; F. Xu; W. Kong and C. L. Jiang
	2015
发表期刊	Vaccine
卷号	33 期号:48 页码:6596-6603
摘要	Enterovirus 71 (EV71) and Coxsackievirus A16 (CVA16), as the main agents causing hand, foot and mouth disease (HFMD), have become a serious public health concern in the Asia-Pacific region. Recently, various neutralizing B cell epitopes of EV71 were identified as targets for promising vaccine candidates. Structural studies of Picornaviridae indicated that potent immunodominant epitopes typically lie in the hypervariable loop of capsid surfaces. However, cross-neutralizing antibodies and cross-protection between EV71 and CVA16 have not been observed. Therefore, we speculated that divergent sequences of the two viruses are key epitopes for inducing protective neutralizing responses. In this study, we selected 10 divergent epitope candidates based on alignment of the EV71 and CVA16 P1 amino acid sequences using the Multalin interface page, and these epitopes are conserved among all subgenotypes of EV71. Simultaneously, by utilizing the norovirus P particle as a novel vaccine delivery carrier, we identified the 71-6 epitope (amino acid 176-190 of VP3) as a conformational neutralizing epitope against EV71 in an in vitro micro-neutralization assay as well as an in vivo protection assay in mice. Altogether, these results indicated that the incorporation of the 71-6 epitope into the norovirus P domain can provide a promising candidate for an effective synthetic peptide-based vaccine against EV71. Crown Copyright (C) 2015 Published by Elsevier Ltd. All rights reserved.
收录类别	SCI
文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/55294
专题	中科院长春光机所知识产出
推荐引用方式 GB/T 7714	Jiang, L. P.,R. J. Fan,S. Y. Sun,et al. A new EV71 VP3 epitope in norovirus P particle vector displays neutralizing activity and protection in vivo in mice[J]. Vaccine,2015,33(48):6596-6603.
APA	Jiang, L. P..,R. J. Fan.,S. Y. Sun.,P. Fan.,W. H. Su.,...&W. Kong and C. L. Jiang.(2015).A new EV71 VP3 epitope in norovirus P particle vector displays neutralizing activity and protection in vivo in mice.Vaccine,33(48),6596-6603.
MLA	Jiang, L. P.,et al."A new EV71 VP3 epitope in norovirus P particle vector displays neutralizing activity and protection in vivo in mice".Vaccine 33.48(2015):6596-6603.

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