Effect of polarization, phase and amplitude ondepletion focus spot in STED

	Effect of polarization, phase and amplitude ondepletion focus spot in STED
	Wei T.-D.; Zhang Y.-H.; Tang Y.-G.
	2014
发表期刊	Guangxue Jingmi Gongcheng/Optics and Precision Engineering
ISSN	ISBN/1004924X
卷号	22 期号:5 页码:1157-1164
摘要	As Stimulated Emission Depletion (STED) super-resolution microscopy has a lower resolution, this paper explores the effects of multiphysical variables including light polarization, phases and amplitudes on the focal spots, in order to form a ring-shape depletion focus spot with sharper Full Width at Half Maximum (FWHM) and to increase the resolution of the microscopy. According to Richards-Wolf vector theory, the models of depletion focus spots were established under the actions of polarization, phase and amplitude, the focus spot distribution was calculated in different polarization, phase and amplitude conditions and the distribution of effective fluorescent excitation was obtained by optimizing the parameters. The results show that: by using azimuthal polarization light as depletion, the FWHM is better than those of radial or circular ones; modulations of phase and amplitude are able to reduce the FWHM, and the theoretical value of effective fluorescent excitation FWHM is only 13.2 nm after optimization. By using polarization state, phase and amplitude to modulate the depletion focus spot, the FWHM can be reduced and a higher resolution can be obtained, which is more effective than that using only single physical variable. Moreover, the high quality depletion focus spot and super-resolution ability can be obtained with azimuthal polarization light in STED and the resolution can further be optimized by phase or amplitude modulations based on different realities.
收录类别	EI
语种	中文
文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/43934
专题	中科院长春光机所知识产出
推荐引用方式 GB/T 7714	Wei T.-D.,Zhang Y.-H.,Tang Y.-G.. Effect of polarization, phase and amplitude ondepletion focus spot in STED[J]. Guangxue Jingmi Gongcheng/Optics and Precision Engineering,2014,22(5):1157-1164.
APA	Wei T.-D.,Zhang Y.-H.,&Tang Y.-G..(2014).Effect of polarization, phase and amplitude ondepletion focus spot in STED.Guangxue Jingmi Gongcheng/Optics and Precision Engineering,22(5),1157-1164.
MLA	Wei T.-D.,et al."Effect of polarization, phase and amplitude ondepletion focus spot in STED".Guangxue Jingmi Gongcheng/Optics and Precision Engineering 22.5(2014):1157-1164.

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