Improvement of the enantioselectivity and activity of lipase from Pseudomonas sp via adsorption on a hydrophobic support: kinetic resolution of 2-octanol

	Improvement of the enantioselectivity and activity of lipase from Pseudomonas sp via adsorption on a hydrophobic support: kinetic resolution of 2-octanol
其他题名	论文其他题名
	Du C.; Zhao B.; Li C. Y.; Wang P.; Wang Z.; Tang J.; Wang L.
	2009
发表期刊	Biocatalysis and Biotransformation
ISSN	1024-2422
卷号	27 期号:5—6 页码:340-347
摘要	Pseudomonas sp. lipase (PSL) was successfully immobilized on a novel hydrophobic polymer support through physical adsorption and the immobilized PSL was used for resolution of (R,S)-2-octanol with vinyl acetate as acyl donor. Enhanced activity and enantioselectivity were observed from the immobilized PSL compared with free PSL. The effects of reaction conditions such as temperature, water activity, substrate molar ratio and the amount of immobilized lipase were investigated. Under optimum conditions, the residual (S)-2-octanol was recovered with 99.5% enantiomeric excess at 52.9% conversion. The results also indicated that the immobilized PSL could maintain 94% of its initial activity even after reusing it five times.
收录类别	SCI ; EI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/26287
专题	中科院长春光机所知识产出
推荐引用方式 GB/T 7714	Du C.,Zhao B.,Li C. Y.,et al. Improvement of the enantioselectivity and activity of lipase from Pseudomonas sp via adsorption on a hydrophobic support: kinetic resolution of 2-octanol[J]. Biocatalysis and Biotransformation,2009,27(5—6):340-347.
APA	Du C..,Zhao B..,Li C. Y..,Wang P..,Wang Z..,...&Wang L..(2009).Improvement of the enantioselectivity and activity of lipase from Pseudomonas sp via adsorption on a hydrophobic support: kinetic resolution of 2-octanol.Biocatalysis and Biotransformation,27(5—6),340-347.
MLA	Du C.,et al."Improvement of the enantioselectivity and activity of lipase from Pseudomonas sp via adsorption on a hydrophobic support: kinetic resolution of 2-octanol".Biocatalysis and Biotransformation 27.5—6(2009):340-347.

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