Binding effect of Tb3+ and Ca2+ ions on trichosanthin

	Binding effect of Tb3+ and Ca2+ ions on trichosanthin
其他题名	论文其他题名
	Liu G. H.; Li W. L.; Hao Q.; Zhao X.; Zhang Y.; Yang H.; Huang Z. L.; Gao G.; Dong Q. C.; Li Q. S.
	1995
发表期刊	Chemical Journal of Chinese Universities-Chinese
ISSN	0251-0790
卷号	16 期号:12 页码:1907-1910
摘要	Tb3+ has been used as a fluorescent probe to study the structure of trichosanthin (TCS), The nonradioactive energy transfer from fluorescent active tryptophan(Trp) residue to the bound Tb3+ ions take place and the fluorescence of Trp is decreased when Tb3+ ions are bound to TCS to replace the Ca2+ in it, According to the results of Trp fluorescence and tl ie information given from CD and UV absorption spectra, we have found that the affinity of Tb3+ to TCS is lower than that of Ca2+ ions to TCS, Significant change of TCS structure was not observed with the Tb3+ ions binding, The microenvironment surrounded Trp and Tyr may be changed slightly with the Tb3+ binding, The conformation of Ca2+-free TCS was significantly changed compared with that oi native TCS with the Trp residue moving into the interior of TCS molecules. The Ca2+-binding site in TCS can be maintained partly when the Ca2+ is removed from TCS molecules.
收录类别	SCI
语种	中文
WOS记录号	WOS:A1995UC19400021
引用统计
文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/25969
专题	中科院长春光机所知识产出
推荐引用方式 GB/T 7714	Liu G. H.,Li W. L.,Hao Q.,et al. Binding effect of Tb3+ and Ca2+ ions on trichosanthin[J]. Chemical Journal of Chinese Universities-Chinese,1995,16(12):1907-1910.
APA	Liu G. H..,Li W. L..,Hao Q..,Zhao X..,Zhang Y..,...&Li Q. S..(1995).Binding effect of Tb3+ and Ca2+ ions on trichosanthin.Chemical Journal of Chinese Universities-Chinese,16(12),1907-1910.
MLA	Liu G. H.,et al."Binding effect of Tb3+ and Ca2+ ions on trichosanthin".Chemical Journal of Chinese Universities-Chinese 16.12(1995):1907-1910.

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