Study of applying fluorescence spectrum imaging to quantitative assay of proteins in bio-chip

	Study of applying fluorescence spectrum imaging to quantitative assay of proteins in bio-chip
其他题名	论文其他题名
	Chao K. F.; Zhang Y. L.; Kong X. G.; Li B.; Zeng Q. H.; Song K.; Sun Y. J.
	2008
发表期刊	Spectroscopy and Spectral Analysis
ISSN	1000-0593
卷号	28 期号:7 页码:1603-1607
摘要	In the present work, the amount and the activity of the goat anti-human IgG, to bind the human IgG labelled with fluorescein isothiocyanate (FITC), immobilized on silicon surfaces modified with APTES and APTES-Glu, respectively, were studied using the fluorescence spectrum imaging(FSI), the results of which were compared with that of ellipsometry. It is shown that the amount of the human IgG labeled with FITC on APTES-Glu measured using FSI is 2. 8 times higher than that on APTES, which is nearly coincident with the 2. 2 times obtained using ellipsometry, showing that the activity of the goat anti-human IgG on APTES-Glu is higher than that on APTES. It is reasoned that the FSI is used in the fluorescence immunoassay the for measurement of quasi-quantification or quantification.
收录类别	SCI
语种	中文
文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/25688
专题	中科院长春光机所知识产出
推荐引用方式 GB/T 7714	Chao K. F.,Zhang Y. L.,Kong X. G.,et al. Study of applying fluorescence spectrum imaging to quantitative assay of proteins in bio-chip[J]. Spectroscopy and Spectral Analysis,2008,28(7):1603-1607.
APA	Chao K. F..,Zhang Y. L..,Kong X. G..,Li B..,Zeng Q. H..,...&Sun Y. J..(2008).Study of applying fluorescence spectrum imaging to quantitative assay of proteins in bio-chip.Spectroscopy and Spectral Analysis,28(7),1603-1607.
MLA	Chao K. F.,et al."Study of applying fluorescence spectrum imaging to quantitative assay of proteins in bio-chip".Spectroscopy and Spectral Analysis 28.7(2008):1603-1607.

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