Changchun Institute of Optics,Fine Mechanics and Physics,CAS
| Ultrasensitive dual-enhanced sandwich strategy for simultaneous detection of Escherichia coli and Staphylococcus aureus based on optimized aptamers-functionalized magnetic capture probes and graphene oxide-Au nanostars SERS tags | |
| W. Zhao, S. Yang, D. Zhang, T. Zhou, J. Huang, M. Gao, Y. Jiang, Y. Liu and J. Yang | |
| 2023 | |
| Source Publication | Journal of Colloid and Interface Science
 |
| ISSN | 00219797 |
| Volume | 634Pages:651-663 |
| Abstract | In this work, a novel surface-enhanced Raman scattering (SERS) sandwich strategy biosensing platform has been established for simultaneously detecting Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). Fe3O4@SiO2-Au nanocomposites (NCs) with varying amounts of Au nanocrystals were prepared, and the effect of interparticle gaps on SERS activity was studied by finite-difference time-domain (FDTD) method. The optimal magnetic SERS-active substrates (FS-A5) were functionalized with the specific aptamers to act as capture probes. Meanwhile, graphene oxide-Au nanostars (GO-Au NSs) decorated with Raman reporters and aptamers were used as SERS tags. The loading density of Au NSs on GO was tuned to change the number of SERS active sites. In this proposal, E. coli and S. aureus were first captured by capture probes and then bound with SERS tags to form a sandwich-like structure, which caused enhanced electromagnetic field because of the dual enhancement strategy. Under optimal conditions, SERS platform could detect E. coli and S. aureus simultaneously, and the detection limit was as low as 10 cfu/mL. Our sandwich assay-based dual-enhanced SERS platform provides a new idea for simultaneously detecting multiple pathogens with high selectivity and sensitivity, and thus will have more hopeful prospects in the field of food safety. © 2022 Elsevier Inc. |
| DOI | 10.1016/j.jcis.2022.12.077 |
| URL | 查看原文 |
| Indexed By | sci ; ei |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ciomp.ac.cn/handle/181722/68238 |
| Collection | 中国科学院长春光学精密机械与物理研究所 |
| Recommended Citation GB/T 7714 | W. Zhao, S. Yang, D. Zhang, T. Zhou, J. Huang, M. Gao, Y. Jiang, Y. Liu and J. Yang. Ultrasensitive dual-enhanced sandwich strategy for simultaneous detection of Escherichia coli and Staphylococcus aureus based on optimized aptamers-functionalized magnetic capture probes and graphene oxide-Au nanostars SERS tags[J]. Journal of Colloid and Interface Science,2023,634:651-663. |
| APA | W. Zhao, S. Yang, D. Zhang, T. Zhou, J. Huang, M. Gao, Y. Jiang, Y. Liu and J. Yang.(2023).Ultrasensitive dual-enhanced sandwich strategy for simultaneous detection of Escherichia coli and Staphylococcus aureus based on optimized aptamers-functionalized magnetic capture probes and graphene oxide-Au nanostars SERS tags.Journal of Colloid and Interface Science,634,651-663. |
| MLA | W. Zhao, S. Yang, D. Zhang, T. Zhou, J. Huang, M. Gao, Y. Jiang, Y. Liu and J. Yang."Ultrasensitive dual-enhanced sandwich strategy for simultaneous detection of Escherichia coli and Staphylococcus aureus based on optimized aptamers-functionalized magnetic capture probes and graphene oxide-Au nanostars SERS tags".Journal of Colloid and Interface Science 634(2023):651-663. |
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| Ultrasensitive dual-（3848KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Download | |
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