中国科学院长春光学精密机械与物理研究所

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	Ultrasensitive dual-enhanced sandwich strategy for simultaneous detection of Escherichia coli and Staphylococcus aureus based on optimized aptamers-functionalized magnetic capture probes and graphene oxide-Au nanostars SERS tags
	W. Zhao, S. Yang, D. Zhang, T. Zhou, J. Huang, M. Gao, Y. Jiang, Y. Liu and J. Yang
	2023
发表期刊	Journal of Colloid and Interface Science
ISSN	00219797
卷号	634页码:651-663
摘要	In this work, a novel surface-enhanced Raman scattering (SERS) sandwich strategy biosensing platform has been established for simultaneously detecting Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). Fe3O4@SiO2-Au nanocomposites (NCs) with varying amounts of Au nanocrystals were prepared, and the effect of interparticle gaps on SERS activity was studied by finite-difference time-domain (FDTD) method. The optimal magnetic SERS-active substrates (FS-A5) were functionalized with the specific aptamers to act as capture probes. Meanwhile, graphene oxide-Au nanostars (GO-Au NSs) decorated with Raman reporters and aptamers were used as SERS tags. The loading density of Au NSs on GO was tuned to change the number of SERS active sites. In this proposal, E. coli and S. aureus were first captured by capture probes and then bound with SERS tags to form a sandwich-like structure, which caused enhanced electromagnetic field because of the dual enhancement strategy. Under optimal conditions, SERS platform could detect E. coli and S. aureus simultaneously, and the detection limit was as low as 10 cfu/mL. Our sandwich assay-based dual-enhanced SERS platform provides a new idea for simultaneously detecting multiple pathogens with high selectivity and sensitivity, and thus will have more hopeful prospects in the field of food safety. © 2022 Elsevier Inc.
DOI	10.1016/j.jcis.2022.12.077
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收录类别	sci ; ei
引用统计
文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/68238
专题	中国科学院长春光学精密机械与物理研究所
推荐引用方式 GB/T 7714	W. Zhao, S. Yang, D. Zhang, T. Zhou, J. Huang, M. Gao, Y. Jiang, Y. Liu and J. Yang. Ultrasensitive dual-enhanced sandwich strategy for simultaneous detection of Escherichia coli and Staphylococcus aureus based on optimized aptamers-functionalized magnetic capture probes and graphene oxide-Au nanostars SERS tags[J]. Journal of Colloid and Interface Science,2023,634:651-663.
APA	W. Zhao, S. Yang, D. Zhang, T. Zhou, J. Huang, M. Gao, Y. Jiang, Y. Liu and J. Yang.(2023).Ultrasensitive dual-enhanced sandwich strategy for simultaneous detection of Escherichia coli and Staphylococcus aureus based on optimized aptamers-functionalized magnetic capture probes and graphene oxide-Au nanostars SERS tags.Journal of Colloid and Interface Science,634,651-663.
MLA	W. Zhao, S. Yang, D. Zhang, T. Zhou, J. Huang, M. Gao, Y. Jiang, Y. Liu and J. Yang."Ultrasensitive dual-enhanced sandwich strategy for simultaneous detection of Escherichia coli and Staphylococcus aureus based on optimized aptamers-functionalized magnetic capture probes and graphene oxide-Au nanostars SERS tags".Journal of Colloid and Interface Science 634(2023):651-663.

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