Bond-selective intensity diffraction tomography

doi:10.1038/s41467-022-35329-8

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	Bond-selective intensity diffraction tomography
	J. Zhao, A. Matlock, H. B. Zhu, Z. Q. Song, J. B. Zhu, B. Wang, F. K. Chen, Y. W. Zhan, Z. C. Chen, Y. H. Xu, X. C. Lin, L. Tian and J. X. Cheng
	2022
发表期刊	Nature Communications
卷号	13 期号:1 页码:12
摘要	Recovering molecular information remains a grand challenge in the widely used holographic and computational imaging technologies. To address this challenge, we developed a computational mid-infrared photothermal microscope, termed Bond-selective Intensity Diffraction Tomography (BS-IDT). Based on a low-cost brightfield microscope with an add-on pulsed light source, BS-IDT recovers both infrared spectra and bond-selective 3D refractive index maps from intensity-only measurements. High-fidelity infrared fingerprint spectra extraction is validated. Volumetric chemical imaging of biological cells is demonstrated at a speed of similar to 20s per volume, with a lateral and axial resolution of similar to 350nm and similar to 1.1 mu m, respectively. BS-IDT's application potential is investigated by chemically quantifying lipids stored in cancer cells and volumetric chemical imaging on Caenorhabditis elegans with a large field of view (similar to 100 mu m x 100 mu m). The authors introduce Bond-selective Intensity Diffraction Tomography, a computational mid-infrared photothermal microscopy technique based on a standard bright-field microscope and an add-on pulsed light source. It recovers both mid-infrared spectra and bond-selective 3D refractive index maps based on intensity-only measurements.
DOI	10.1038/s41467-022-35329-8
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收录类别	sci
语种	英语
引用统计
文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/68231
专题	中国科学院长春光学精密机械与物理研究所
推荐引用方式 GB/T 7714	J. Zhao, A. Matlock, H. B. Zhu, Z. Q. Song, J. B. Zhu, B. Wang, F. K. Chen, Y. W. Zhan, Z. C. Chen, Y. H. Xu, X. C. Lin, L. Tian and J. X. Cheng. Bond-selective intensity diffraction tomography[J]. Nature Communications,2022,13(1):12.
APA	J. Zhao, A. Matlock, H. B. Zhu, Z. Q. Song, J. B. Zhu, B. Wang, F. K. Chen, Y. W. Zhan, Z. C. Chen, Y. H. Xu, X. C. Lin, L. Tian and J. X. Cheng.(2022).Bond-selective intensity diffraction tomography.Nature Communications,13(1),12.
MLA	J. Zhao, A. Matlock, H. B. Zhu, Z. Q. Song, J. B. Zhu, B. Wang, F. K. Chen, Y. W. Zhan, Z. C. Chen, Y. H. Xu, X. C. Lin, L. Tian and J. X. Cheng."Bond-selective intensity diffraction tomography".Nature Communications 13.1(2022):12.

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