Mid-infrared chemical imaging of intracellular tau fibrils using fluorescence-guided computational photothermal microscopy

doi:10.1038/s41377-023-01191-6

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	Mid-infrared chemical imaging of intracellular tau fibrils using fluorescence-guided computational photothermal microscopy
	J. Zhao, L. Jiang, A. Matlock, Y. Xu, J. Zhu, H. Zhu, L. Tian, B. Wolozin and J.-X. Cheng
	2023
发表期刊	Light: Science and Applications
ISSN	20955545
卷号	12 期号:1
摘要	Amyloid proteins are associated with a broad spectrum of neurodegenerative diseases. However, it remains a grand challenge to extract molecular structure information from intracellular amyloid proteins in their native cellular environment. To address this challenge, we developed a computational chemical microscope integrating 3D mid-infrared photothermal imaging with fluorescence imaging, termed Fluorescence-guided Bond-Selective Intensity Diffraction Tomography (FBS-IDT). Based on a low-cost and simple optical design, FBS-IDT enables chemical-specific volumetric imaging and 3D site-specific mid-IR fingerprint spectroscopic analysis of tau fibrils, an important type of amyloid protein aggregates, in their intracellular environment. Label-free volumetric chemical imaging of human cells with/without seeded tau fibrils is demonstrated to show the potential correlation between lipid accumulation and tau aggregate formation. Depth-resolved mid-infrared fingerprint spectroscopy is performed to reveal the protein secondary structure of the intracellular tau fibrils. 3D visualization of the β-sheet for tau fibril structure is achieved. © 2023, The Author(s).
DOI	10.1038/s41377-023-01191-6
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收录类别	sci ; ei
引用统计
文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/68230
专题	中国科学院长春光学精密机械与物理研究所
推荐引用方式 GB/T 7714	J. Zhao, L. Jiang, A. Matlock, Y. Xu, J. Zhu, H. Zhu, L. Tian, B. Wolozin and J.-X. Cheng. Mid-infrared chemical imaging of intracellular tau fibrils using fluorescence-guided computational photothermal microscopy[J]. Light: Science and Applications,2023,12(1).
APA	J. Zhao, L. Jiang, A. Matlock, Y. Xu, J. Zhu, H. Zhu, L. Tian, B. Wolozin and J.-X. Cheng.(2023).Mid-infrared chemical imaging of intracellular tau fibrils using fluorescence-guided computational photothermal microscopy.Light: Science and Applications,12(1).
MLA	J. Zhao, L. Jiang, A. Matlock, Y. Xu, J. Zhu, H. Zhu, L. Tian, B. Wolozin and J.-X. Cheng."Mid-infrared chemical imaging of intracellular tau fibrils using fluorescence-guided computational photothermal microscopy".Light: Science and Applications 12.1(2023).

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