Changchun Institute of Optics,Fine Mechanics and Physics,CAS
Mid-infrared chemical imaging of intracellular tau fibrils using fluorescence-guided computational photothermal microscopy | |
J. Zhao, L. Jiang, A. Matlock, Y. Xu, J. Zhu, H. Zhu, L. Tian, B. Wolozin and J.-X. Cheng | |
2023 | |
发表期刊 | Light: Science and Applications
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ISSN | 20955545 |
卷号 | 12期号:1 |
摘要 | Amyloid proteins are associated with a broad spectrum of neurodegenerative diseases. However, it remains a grand challenge to extract molecular structure information from intracellular amyloid proteins in their native cellular environment. To address this challenge, we developed a computational chemical microscope integrating 3D mid-infrared photothermal imaging with fluorescence imaging, termed Fluorescence-guided Bond-Selective Intensity Diffraction Tomography (FBS-IDT). Based on a low-cost and simple optical design, FBS-IDT enables chemical-specific volumetric imaging and 3D site-specific mid-IR fingerprint spectroscopic analysis of tau fibrils, an important type of amyloid protein aggregates, in their intracellular environment. Label-free volumetric chemical imaging of human cells with/without seeded tau fibrils is demonstrated to show the potential correlation between lipid accumulation and tau aggregate formation. Depth-resolved mid-infrared fingerprint spectroscopy is performed to reveal the protein secondary structure of the intracellular tau fibrils. 3D visualization of the β-sheet for tau fibril structure is achieved. © 2023, The Author(s). |
DOI | 10.1038/s41377-023-01191-6 |
URL | 查看原文 |
收录类别 | sci ; ei |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.ciomp.ac.cn/handle/181722/68230 |
专题 | 中国科学院长春光学精密机械与物理研究所 |
推荐引用方式 GB/T 7714 | J. Zhao, L. Jiang, A. Matlock, Y. Xu, J. Zhu, H. Zhu, L. Tian, B. Wolozin and J.-X. Cheng. Mid-infrared chemical imaging of intracellular tau fibrils using fluorescence-guided computational photothermal microscopy[J]. Light: Science and Applications,2023,12(1). |
APA | J. Zhao, L. Jiang, A. Matlock, Y. Xu, J. Zhu, H. Zhu, L. Tian, B. Wolozin and J.-X. Cheng.(2023).Mid-infrared chemical imaging of intracellular tau fibrils using fluorescence-guided computational photothermal microscopy.Light: Science and Applications,12(1). |
MLA | J. Zhao, L. Jiang, A. Matlock, Y. Xu, J. Zhu, H. Zhu, L. Tian, B. Wolozin and J.-X. Cheng."Mid-infrared chemical imaging of intracellular tau fibrils using fluorescence-guided computational photothermal microscopy".Light: Science and Applications 12.1(2023). |
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文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
Mid-infrared chemica(6722KB) | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 |
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