A fully automated Lab-on-a-Disc platform integrated a high-speed triggered siphon valve for PBMCs extraction

doi:10.1016/j.talanta.2023.125292

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	A fully automated Lab-on-a-Disc platform integrated a high-speed triggered siphon valve for PBMCs extraction
	J. H. Zhang, J. Y. Ma, Y. Xu, Y. H. Wu and M. S. Miao
	2024
Source Publication	Talanta
ISSN	0039-9140
Volume	268 Pages:8
Abstract	Human Peripheral Blood Mononuclear Cells (PBMCs) are isolated from peripheral blood and identified as any blood cell with a round nucleus that exhibits immune responses and undergoes immunophenotypic changes upon exposure to various pathophysiological stimuli. Obtaining high-recovery and clinical-grade PBMCs without decreasing cell viability and causing stress is crucial for disease diagnosis and successful immunotherapy. However, traditional manual PBMCs extraction methods rely on manual intervention with less recovery rate and reliability. In this study, we introduced a novel and efficient strategy for the fully automated extraction of PBMCs based on a Lab-on-a-Disk (LoaD) platform. The centrifugal chip used percoll as density gradient media (DGM) for separation and extraction on account of the density difference of cells in whole blood, without labeling and any additional extra cellular filtration or cell lysis steps. Above all, we proposed a high-speed triggered siphon valve, which was closed under the speed of cell sedimentation and subsequently opened by increasing speed to complete the extraction of PBMCs. It can avoid the problem that previous siphon valves rely on unstable hydrophilic surface treatment and prime under low/zero speed conditions. With valves and the clock channel integrated on the chip, users can achieve fully automated collection of PBMCs. Compared with the clinical laboratory results, the recovery rate of extracted PBMCs was 80 %. The experimental results prove that the high-speed triggered siphon valve improves the extraction efficiency of PBMCs. The robust chips, which are not only simple to manufacture and assemble but also stable and reliable to use, have great potential in biomedical and clinical applications.
DOI	10.1016/j.talanta.2023.125292
URL	查看原文
Indexed By	sci
Language	英语
Citation statistics
Document Type	期刊论文
Identifier	http://ir.ciomp.ac.cn/handle/181722/68177
Collection	中国科学院长春光学精密机械与物理研究所
Recommended Citation GB/T 7714	J. H. Zhang, J. Y. Ma, Y. Xu, Y. H. Wu and M. S. Miao. A fully automated Lab-on-a-Disc platform integrated a high-speed triggered siphon valve for PBMCs extraction[J]. Talanta,2024,268:8.
APA	J. H. Zhang, J. Y. Ma, Y. Xu, Y. H. Wu and M. S. Miao.(2024).A fully automated Lab-on-a-Disc platform integrated a high-speed triggered siphon valve for PBMCs extraction.Talanta,268,8.
MLA	J. H. Zhang, J. Y. Ma, Y. Xu, Y. H. Wu and M. S. Miao."A fully automated Lab-on-a-Disc platform integrated a high-speed triggered siphon valve for PBMCs extraction".Talanta 268(2024):8.

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中国科学院长春光学精密机械与物理研究所