Changchun Institute of Optics,Fine Mechanics and Physics,CAS
| Electron coupling effect-triggered monatomic copper laccase-mimicking nanozyme for the degradation and detection of guaiacol produced by Alicyclobacillus acidoterrestris | |
| X. D. Niu, L. F. Wu, F. L. Wu, J. Q. Guan and H. S. Wang | |
| 2023 | |
| 发表期刊 | Biosensors & Bioelectronics
 |
| ISSN | 0956-5663 |
| 卷号 | 238页码:11 |
| 摘要 | The generation of guaiacol by Alicyclobacillus acidoterrestris (A. acidoterrestris) in fruit juices negatively affects public health and causes severe environmental pollution. Therefore, the sensitive detection and efficient degradation of guaiacol in real samples are crucial. Here, we develop an electrochemical sensor utilizing a copper single-atom nanozyme (CuN4-G) to detect and degrade guaiacol at the picomolar level. Density functional theory (DFT) calculations verify that the bonding electron coupling effect in the CuN4-G facilitates rapid electron transfer, enhances electrical conductivity, and provides abundant active sites, thereby leading to exceptional catalytic performance. Moreover, CuN4-G demonstrates a Km value similar to that of natural laccase but a higher Vmax, highlighting its potential as a highly efficient biocatalyst. The CuN4-G-based electrochemical sensor achieves a detection from 5 to 50,000 pM for guaiacol, with a 1.2 pM (S/N = 3) detection limit. Additionally, CuN4- G-modified electrodes display high selectivity and excellent stability. CuN4-G nanozyme can keep its activity in conditions of pH (3-9), temperature (30-90 degrees C), ionic strength (0-400 mM), and organic solvent (0-50% (v/v)), overcoming the deficiencies of natural enzymes. Furthermore, our electrochemical sensor can not only accurately detect guaiacol, but also degrade it in actual fruit juice samples infected by A. acidoterrestris, demonstrating its potential applications in food and environmental monitoring. |
| DOI | 10.1016/j.bios.2023.115606 |
| URL | 查看原文 |
| 收录类别 | sci |
| 语种 | 英语 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ciomp.ac.cn/handle/181722/67780 |
| 专题 | 中国科学院长春光学精密机械与物理研究所 |
| 推荐引用方式 GB/T 7714 | X. D. Niu, L. F. Wu, F. L. Wu, J. Q. Guan and H. S. Wang. Electron coupling effect-triggered monatomic copper laccase-mimicking nanozyme for the degradation and detection of guaiacol produced by Alicyclobacillus acidoterrestris[J]. Biosensors & Bioelectronics,2023,238:11. |
| APA | X. D. Niu, L. F. Wu, F. L. Wu, J. Q. Guan and H. S. Wang.(2023).Electron coupling effect-triggered monatomic copper laccase-mimicking nanozyme for the degradation and detection of guaiacol produced by Alicyclobacillus acidoterrestris.Biosensors & Bioelectronics,238,11. |
| MLA | X. D. Niu, L. F. Wu, F. L. Wu, J. Q. Guan and H. S. Wang."Electron coupling effect-triggered monatomic copper laccase-mimicking nanozyme for the degradation and detection of guaiacol produced by Alicyclobacillus acidoterrestris".Biosensors & Bioelectronics 238(2023):11. |
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| Electron coupling ef（8921KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 下载 | |
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