Multistage microfluidic cell sorting method and chip based on size and stiffness

doi:10.1016/j.bios.2023.115451

CIOMP OpenIR

	Multistage microfluidic cell sorting method and chip based on size and stiffness
	G. Li; Y. Ji; Y. Wu; Y. Liu; H. Li; Y. Wang; M. Chi; H. Sun and H. Zhu
	2023
发表期刊	Biosensors and Bioelectronics
ISSN	09565663
卷号	237
摘要	High performance sorting of circulating tumor cells (CTCs) from peripheral blood is key to liquid biopsies. Size-based deterministic lateral displacement (DLD) technique is widely used in cell sorting. But conventional microcolumns have poor fluid regulation ability, which limits the sorting performance of DLD. When the size difference between CTCs and leukocytes is small (e.g., less than 3 μm), not only DLD, many size-based separation techniques fail due to low specificity. CTCs have been confirmed to be softer than leukocytes, which could serve as a basis for sorting. In this study, we presented a multistage microfluidic CTCs sorting method, first sorting CTCs using a size-based two-array DLD chip, then purifying CTCs mixed by leukocytes using a stiffness-based cone channel chip, and finally identifying cell types using Raman techniques. The entire CTCs sorting and analysis process was label free, highly pure, high-throughput and efficient. The two-array DLD chip employed a droplet-shaped microcolumn (DMC) developed by optimization design rather than empirical design. Attributed to the excellent fluid regulation capability of DMC, the CTCs sorter system developed by parallelizing four DMC two-array DLD chips was able to process a sample of 2.5 mL per minute with a recovery efficiency of 96.30 ± 2.10% and a purity of 98.25 ± 2.48%. To isolate CTCs mixed dimensionally by leukocytes, a cone channel sorting method and chip were developed based on solid and hydrodynamic coupled analysis. The cone channel chip allowed CTCs to pass through the channel and entrap leukocytes, improving the purity of CTCs mixed by leukocytes by 1.8-fold. © 2023 Elsevier B.V.
DOI	10.1016/j.bios.2023.115451
URL	查看原文
收录类别	sci ; ei
引用统计
文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/67605
专题	中国科学院长春光学精密机械与物理研究所
推荐引用方式 GB/T 7714	G. Li,Y. Ji,Y. Wu,et al. Multistage microfluidic cell sorting method and chip based on size and stiffness[J]. Biosensors and Bioelectronics,2023,237.
APA	G. Li.,Y. Ji.,Y. Wu.,Y. Liu.,H. Li.,...&H. Sun and H. Zhu.(2023).Multistage microfluidic cell sorting method and chip based on size and stiffness.Biosensors and Bioelectronics,237.
MLA	G. Li,et al."Multistage microfluidic cell sorting method and chip based on size and stiffness".Biosensors and Bioelectronics 237(2023).

条目包含的文件		下载所有文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Multistage microflui（13036KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览下载