Phycocyanin - carbon dots nanoprobe for the ratiometric fluorescence determination of peroxynitrite

doi:10.1016/j.saa.2022.121177

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	Phycocyanin - carbon dots nanoprobe for the ratiometric fluorescence determination of peroxynitrite
	J. J. Shao; S. Sun; D. Zhan; L. P. Pan; G. Z. Min; X. B. Li; K. L. Huang; W. Chen; L. K. Yang; X. Y. Liu and N. B. Lin
	2022
发表期刊	Spectrochimica Acta Part a-Molecular and Biomolecular Spectroscopy
ISSN	1386-1425
卷号	275 页码:8
摘要	As a kind of reactive oxygen species, peroxynitrite is related to various diseases closely such as cancer and neurodegenerative diseases. Constructing probes with highly specific ability and a wide linear detection range for peroxynitrite detection is crucial for understanding the pathogenesis of related diseases and optimizing treatments. In this work, we developed a novel luminescent ratiometric fluorescence nanoprobe (PC-CDs) based on carbon dots and phycocyanin. PC-CDs are constructed by amidation reaction between carbon dots and phycocyanin. The nanoprobe we obtained has a good ability of distinguishing peroxynitrite from other reactive oxygen species and interfering substances. Moreover, the linear range of the nanoprobe is 0.5-100 mu M and the limit of detection is 0.5 mu M when detecting peroxynitrite. In the spiked recovery experiments under phosphate buffered saline (PBS) environment, our nanoprobe has a good recovery performance and the recovery is 99% - 104%, which will be beneficial to the further development of peroxynitrite testing and the research progress of related diseases. Finally, we discuss the quenching mechanism of peroxynitrite for nanoprobe, and found that there is the combination of dynamic and static quenching in the quenching process. (C) 2022 Published by Elsevier B.V.
DOI	10.1016/j.saa.2022.121177
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收录类别	sci ; ei
语种	英语
引用统计
文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/66988
专题	中国科学院长春光学精密机械与物理研究所
推荐引用方式 GB/T 7714	J. J. Shao,S. Sun,D. Zhan,et al. Phycocyanin - carbon dots nanoprobe for the ratiometric fluorescence determination of peroxynitrite[J]. Spectrochimica Acta Part a-Molecular and Biomolecular Spectroscopy,2022,275:8.
APA	J. J. Shao.,S. Sun.,D. Zhan.,L. P. Pan.,G. Z. Min.,...&X. Y. Liu and N. B. Lin.(2022).Phycocyanin - carbon dots nanoprobe for the ratiometric fluorescence determination of peroxynitrite.Spectrochimica Acta Part a-Molecular and Biomolecular Spectroscopy,275,8.
MLA	J. J. Shao,et al."Phycocyanin - carbon dots nanoprobe for the ratiometric fluorescence determination of peroxynitrite".Spectrochimica Acta Part a-Molecular and Biomolecular Spectroscopy 275(2022):8.

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