An Optimized Thermal Feedback Methodology for Accurate Temperature Control and High Amplification Efficiency during Fluorescent qPCR

doi:10.3390/bioengineering9060237

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	An Optimized Thermal Feedback Methodology for Accurate Temperature Control and High Amplification Efficiency during Fluorescent qPCR
	K. N. Wang; Y. Y. Jiang; Y. Guo; M. K. Geng and W. M. Wu
	2022
发表期刊	Bioengineering-Basel
卷号	9 期号:6 页码:9
摘要	Traditional qPCR instrument is combined with CMOS and a personal computer, and a photoelectric feedback automatic fluorescence detection system is designed to realize quantitative real-time PCR. The key to reaction efficiency lies in how to ensure that the temperature of the detection reagent completely matches the set temperature. However, for most traditional real-time fluorescent PCR systems, the temperature cycling is controlled by detecting the temperature of the heating well plate. It cannot directly measure the temperature in the reaction reagent PCR tube, which will cause the deviation in the actual temperature of the reagent to be as expected. Therefore, in this paper, we raise a method of directly detecting the temperature in the reaction tube of the reagent during the temperature cycling is adopted. According to the deviation from the expected value, the set temperature of the PCR instrument is adjusted to make the actual temperature of the reagent closer to the expected value. Through this method, we also realized the temperature calibration and optimization of the TEC circulation system we built. Experiments show that this low-cost, portable real-time quantitative PCR system can detect and analyze pathogens in situ.
DOI	10.3390/bioengineering9060237
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收录类别	sci
语种	英语
引用统计
文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/66958
专题	中国科学院长春光学精密机械与物理研究所
推荐引用方式 GB/T 7714	K. N. Wang,Y. Y. Jiang,Y. Guo,et al. An Optimized Thermal Feedback Methodology for Accurate Temperature Control and High Amplification Efficiency during Fluorescent qPCR[J]. Bioengineering-Basel,2022,9(6):9.
APA	K. N. Wang,Y. Y. Jiang,Y. Guo,&M. K. Geng and W. M. Wu.(2022).An Optimized Thermal Feedback Methodology for Accurate Temperature Control and High Amplification Efficiency during Fluorescent qPCR.Bioengineering-Basel,9(6),9.
MLA	K. N. Wang,et al."An Optimized Thermal Feedback Methodology for Accurate Temperature Control and High Amplification Efficiency during Fluorescent qPCR".Bioengineering-Basel 9.6(2022):9.

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