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A novel cascade signal amplification strategy integrating CRISPR/Cas13a and branched hybridization chain reaction for ultra-sensitive and specific SERS detection of disease-related nucleic acids
J. J. Zhang; C. Y. Song; Y. F. Zhu; H. Y. Gan; X. Y. Fang; Q. Peng; J. R. Xiong; C. Dong; C. Q. Han and L. H. Wang
2023
Source PublicationBiosensors & Bioelectronics
ISSN0956-5663
Volume219Pages:10
AbstractThe molecular diagnosis of disease by high-sensitively and specifically detecting extremely trace amounts of nucleic acid biomarkers in biological samples is still a great challenge, and the powerful sensing strategy has become an urgent need for basic researches and clinical applications. Herein, a novel one-pot cascade signal amplification strategy (Cas13a-bHCR) integrating CRISPR/Cas13a system (Cas13a) and branched hybridization chain reaction (bHCR) was proposed for ultra-highly sensitive and specific SERS assay of disease-related nucleic acids on SERS-active silver nanorods sensing chips. The Cas13a-bHCR based SERS assay of gastric cancer-related miRNA-106a (miR-106a) can be achieved within 60 min and output significantly enhanced SERS signal due to the multiple signal amplification, which possesses a good linear calibration curve from 10 aM to 1 nM with the limit of detection (LOD) low to 8.55 aM for detecting gastric cancer-related miR-106a in human serum. The Cas13a-bHCR based SERS sensing also shows good specificity, uniformity, repeatability and reliability, and has good practicability for detection of miR-106a in clinical samples, which can provide a potential powerful tool for SERS detection of disease-related nucleic acids and promise brighter prospects in the field of clinical diagnosis of early disease.
DOI10.1016/j.bios.2022.114836
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Indexed Bysci
Language英语
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Document Type期刊论文
Identifierhttp://ir.ciomp.ac.cn/handle/181722/66911
Collection中国科学院长春光学精密机械与物理研究所
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GB/T 7714
J. J. Zhang,C. Y. Song,Y. F. Zhu,et al. A novel cascade signal amplification strategy integrating CRISPR/Cas13a and branched hybridization chain reaction for ultra-sensitive and specific SERS detection of disease-related nucleic acids[J]. Biosensors & Bioelectronics,2023,219:10.
APA J. J. Zhang.,C. Y. Song.,Y. F. Zhu.,H. Y. Gan.,X. Y. Fang.,...&C. Q. Han and L. H. Wang.(2023).A novel cascade signal amplification strategy integrating CRISPR/Cas13a and branched hybridization chain reaction for ultra-sensitive and specific SERS detection of disease-related nucleic acids.Biosensors & Bioelectronics,219,10.
MLA J. J. Zhang,et al."A novel cascade signal amplification strategy integrating CRISPR/Cas13a and branched hybridization chain reaction for ultra-sensitive and specific SERS detection of disease-related nucleic acids".Biosensors & Bioelectronics 219(2023):10.
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