The Effects of Optical Aberrations to Illumination Beam Thickness in Two-Photon Excitation Microscopes

doi:10.3390/app12147156

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	The Effects of Optical Aberrations to Illumination Beam Thickness in Two-Photon Excitation Microscopes
	N. Li; F. L. Luo; C. L. Yang; Z. H. Peng; L. Xuan; Q. P. Bu; Q. Q. Mu and X. Y. Zhang
	2022
发表期刊	Applied Sciences-Basel
卷号	12 期号:14 页码:12
摘要	When performing in vivo imaging of live samples, it is a big challenge to penetrate thick tissues while still maintaining high resolution and a large field of view because of the sample-induced aberrations. These requirements can be met by combining the benefits of two-photon excitation, beam modulation and adaptive optics in an illumination path. However, the relationship between aberrations and the performance of such a microscopy system has never been systematically and comprehensively assessed. Here, two-photon Gaussian and Bessel beams are modulated as illumination beams, and how aberrations affect the thickness of the illumination beams is evaluated. It is found that the thickness variation is highly related to the azimuthal order of Zernike modes. The thickness of the two-photon Gaussian beam is more sensitive to Zernike modes with lower azimuthal order, while the thickness of the two-photon Bessel beam is more sensitive to the higher-azimuthal-order Zernike modes. So, it is necessary to design a new strategy to correct aberrations according to the effects of different Zernike modes in order to maximize the correction capability of correctors and reduce the correction errors for those insensitive Zernike modes. These results may provide important guidance for the design and evaluation of adaptive optical systems in a two-photon excitation microscope.
DOI	10.3390/app12147156
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收录类别	sci
语种	英语
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文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/66599
专题	中国科学院长春光学精密机械与物理研究所
推荐引用方式 GB/T 7714	N. Li,F. L. Luo,C. L. Yang,et al. The Effects of Optical Aberrations to Illumination Beam Thickness in Two-Photon Excitation Microscopes[J]. Applied Sciences-Basel,2022,12(14):12.
APA	N. Li.,F. L. Luo.,C. L. Yang.,Z. H. Peng.,L. Xuan.,...&Q. Q. Mu and X. Y. Zhang.(2022).The Effects of Optical Aberrations to Illumination Beam Thickness in Two-Photon Excitation Microscopes.Applied Sciences-Basel,12(14),12.
MLA	N. Li,et al."The Effects of Optical Aberrations to Illumination Beam Thickness in Two-Photon Excitation Microscopes".Applied Sciences-Basel 12.14(2022):12.

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