Construction of dPCR and qPCR integrated system based on commercially available low-cost hardware

doi:10.1039/d2an00694d

CIOMP OpenIR

	Construction of dPCR and qPCR integrated system based on commercially available low-cost hardware
	K. N. Wang; B. L. Sang; L. M. He; Y. Guo; M. K. Geng; D. Z. Zheng; X. L. Xu and W. M. Wu
	2022
发表期刊	Analyst
ISSN	0003-2654
卷号	147 期号:15 页码:3494-3503
摘要	Fluorescent quantitative PCR (qPCR) and digital PCR (dPCR) are two mainstream nucleic acid quantification technologies. However, commercial dPCR and qPCR instruments have a low integration, a high price, and a large footprint. To solve these shortcomings, we introduce a compound PCR system with both qPCR and dPCR functions. All the hardware used in this compound PCR system is commercially available and low-cost, and free software was used to realize the absolute quantification of nucleic acids. The compound PCR provides two working modes. In the qPCR mode, thermal cycling is realized by controlling the reciprocating motion of the x axis. The heating rate is 1.25 degrees C s(-1) and the cooling rate is 1.75 degrees C s(-1). We performed amplification experiments of the PGEM-3zf (+)1 gene. The performance level was similar to commercial qPCR instruments. In the dPCR mode, the heating rate is 0.5 degrees C s(-1) and the cooling rate is 0.6 degrees C s(-1). We performed the UPE-Q gene amplification and used the sequential actions of the two-dimensional mechanical sliders to scan the reaction products and used the method of regional statistics and back-inference threshold to get test results. The result we got was 1208 copies per mu L-1, which was similar to expectations.
DOI	10.1039/d2an00694d
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收录类别	sci ; ei
语种	英语
引用统计
文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/66486
专题	中国科学院长春光学精密机械与物理研究所
推荐引用方式 GB/T 7714	K. N. Wang,B. L. Sang,L. M. He,et al. Construction of dPCR and qPCR integrated system based on commercially available low-cost hardware[J]. Analyst,2022,147(15):3494-3503.
APA	K. N. Wang.,B. L. Sang.,L. M. He.,Y. Guo.,M. K. Geng.,...&X. L. Xu and W. M. Wu.(2022).Construction of dPCR and qPCR integrated system based on commercially available low-cost hardware.Analyst,147(15),3494-3503.
MLA	K. N. Wang,et al."Construction of dPCR and qPCR integrated system based on commercially available low-cost hardware".Analyst 147.15(2022):3494-3503.

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