Changchun Institute of Optics,Fine Mechanics and Physics,CAS
Biphenyl-degrading Bacteria Isolation with Laser Induced Visualized Ejection Separation Technology and Traditional Colony Sorting | |
A. L. Li; K. Z. Chen; B. Li; P. Liang and C. F. Shen | |
2022 | |
发表期刊 | Bulletin of Environmental Contamination and Toxicology |
ISSN | 0007-4861 |
卷号 | 109期号:3页码:571-576 |
摘要 | In this work, biphenyl was used as carbon source to enrich microorganisms from polychlorinated biphenyls (PCBs)-contaminated paddy soil samples, and the taxonomic structures in both of the soil samples and the fourth-generation enrichments were examined with high-throughput sequencing. Single cells were isolated from the enrichments via single cell sorting technology named Laser Induced Visualized Ejection Separation Technology (LIVEST) and also traditional single colony sorting, and the genera of the isolates were identified using 16S rRNA sequencing. The results from high-throughput sequencing present that enrichment from generation to generation can considerably change the microbial community. Comparing the two sorting methods, the LIVEST is more time-saving and cell-targeted for microbial resource exploration. Based on the further verification of biphenyl degradation, it was found that some strains belonging to genera Macrococcus, Aerococcus and Metabacillus are capable in degrading biphenyl, which have not been reported yet. |
DOI | 10.1007/s00128-022-03574-8 |
URL | 查看原文 |
收录类别 | sci |
语种 | 英语 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.ciomp.ac.cn/handle/181722/66441 |
专题 | 中国科学院长春光学精密机械与物理研究所 |
推荐引用方式 GB/T 7714 | A. L. Li,K. Z. Chen,B. Li,et al. Biphenyl-degrading Bacteria Isolation with Laser Induced Visualized Ejection Separation Technology and Traditional Colony Sorting[J]. Bulletin of Environmental Contamination and Toxicology,2022,109(3):571-576. |
APA | A. L. Li,K. Z. Chen,B. Li,&P. Liang and C. F. Shen.(2022).Biphenyl-degrading Bacteria Isolation with Laser Induced Visualized Ejection Separation Technology and Traditional Colony Sorting.Bulletin of Environmental Contamination and Toxicology,109(3),571-576. |
MLA | A. L. Li,et al."Biphenyl-degrading Bacteria Isolation with Laser Induced Visualized Ejection Separation Technology and Traditional Colony Sorting".Bulletin of Environmental Contamination and Toxicology 109.3(2022):571-576. |
条目包含的文件 | 下载所有文件 | |||||
文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
Biphenyl-degrading B(992KB) | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 下载 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论