Biphenyl-degrading Bacteria Isolation with Laser Induced Visualized Ejection Separation Technology and Traditional Colony Sorting

doi:10.1007/s00128-022-03574-8

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	Biphenyl-degrading Bacteria Isolation with Laser Induced Visualized Ejection Separation Technology and Traditional Colony Sorting
	A. L. Li; K. Z. Chen; B. Li; P. Liang and C. F. Shen
	2022
发表期刊	Bulletin of Environmental Contamination and Toxicology
ISSN	0007-4861
卷号	109 期号:3 页码:571-576
摘要	In this work, biphenyl was used as carbon source to enrich microorganisms from polychlorinated biphenyls (PCBs)-contaminated paddy soil samples, and the taxonomic structures in both of the soil samples and the fourth-generation enrichments were examined with high-throughput sequencing. Single cells were isolated from the enrichments via single cell sorting technology named Laser Induced Visualized Ejection Separation Technology (LIVEST) and also traditional single colony sorting, and the genera of the isolates were identified using 16S rRNA sequencing. The results from high-throughput sequencing present that enrichment from generation to generation can considerably change the microbial community. Comparing the two sorting methods, the LIVEST is more time-saving and cell-targeted for microbial resource exploration. Based on the further verification of biphenyl degradation, it was found that some strains belonging to genera Macrococcus, Aerococcus and Metabacillus are capable in degrading biphenyl, which have not been reported yet.
DOI	10.1007/s00128-022-03574-8
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收录类别	sci
语种	英语
引用统计
文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/66441
专题	中国科学院长春光学精密机械与物理研究所
推荐引用方式 GB/T 7714	A. L. Li,K. Z. Chen,B. Li,et al. Biphenyl-degrading Bacteria Isolation with Laser Induced Visualized Ejection Separation Technology and Traditional Colony Sorting[J]. Bulletin of Environmental Contamination and Toxicology,2022,109(3):571-576.
APA	A. L. Li,K. Z. Chen,B. Li,&P. Liang and C. F. Shen.(2022).Biphenyl-degrading Bacteria Isolation with Laser Induced Visualized Ejection Separation Technology and Traditional Colony Sorting.Bulletin of Environmental Contamination and Toxicology,109(3),571-576.
MLA	A. L. Li,et al."Biphenyl-degrading Bacteria Isolation with Laser Induced Visualized Ejection Separation Technology and Traditional Colony Sorting".Bulletin of Environmental Contamination and Toxicology 109.3(2022):571-576.

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