Visualizing and Isolating Iron-Reducing Microorganisms at the Single-Cell Level

doi:10.1128/AEM.02192-20

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	Visualizing and Isolating Iron-Reducing Microorganisms at the Single-Cell Level
	C. Gan; R. Wu; Y. Luo; J. Song; D. Luo; B. Li; Y. Yang and M. Xu
	2021
发表期刊	Applied and Environmental Microbiology
ISSN	992240
卷号	87 期号:3 页码:1-11
摘要	Iron-reducing microorganisms (FeRM) play key roles in many natural and engineering processes. Visualizing and isolating FeRM from multispecies samples are essential to understand the in situ location and geochemical role of FeRM. Here, we visualized FeRM by a "turn-on" Fe21-specific fluorescent chemodosimeter (FSFC) with high sensitivity, selectivity, and stability. This FSFC could selectively identify and locate active FeRM from either pure culture, coculture of different bacteria, or sediment-containing samples. Fluorescent intensity of the FSFC could be used as an indicator of Fe21 concentration in bacterial cultures. By combining the use of the FSFC with that of a single-cell sorter, we obtained three FSFC-labeled cells from an enriched consortium, and all of them were subsequently shown to be capable of iron reduction; two unlabeled cells were shown to have no iron-reducing capability, further confirming the feasibility of the FSFC. IMPORTANCE Visualization and isolation of FeRM from samples containing multiple species are commonly needed by researchers from different disciplines, such as environmental microbiology, environmental sciences, and geochemistry. However, no available method has been reported. In this study, we provide a method to visualize FeRM and evaluate their activity even at the single-cell level. When this approach is combined with use of a single-cell sorter, FeRM can also be isolated from samples containing multiple species. This method can be used as a powerful tool to uncover the in situ or ex situ role of FeRM and their interactions with ambient microbes or chemicals. 2021. American Society for Microbiology. All Rights Reserved.
DOI	10.1128/AEM.02192-20
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收录类别	SCI ; EI
引用统计
文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/65747
专题	中国科学院长春光学精密机械与物理研究所
推荐引用方式 GB/T 7714	C. Gan,R. Wu,Y. Luo,et al. Visualizing and Isolating Iron-Reducing Microorganisms at the Single-Cell Level[J]. Applied and Environmental Microbiology,2021,87(3):1-11.
APA	C. Gan.,R. Wu.,Y. Luo.,J. Song.,D. Luo.,...&Y. Yang and M. Xu.(2021).Visualizing and Isolating Iron-Reducing Microorganisms at the Single-Cell Level.Applied and Environmental Microbiology,87(3),1-11.
MLA	C. Gan,et al."Visualizing and Isolating Iron-Reducing Microorganisms at the Single-Cell Level".Applied and Environmental Microbiology 87.3(2021):1-11.

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