Promotion of rs3746804 (p. L267P) polymorphism to intracellular SLC52A3a trafficking and riboflavin transportation in esophageal cancer cells

doi:10.1007/s00726-021-03025-4

CIOMP OpenIR

	Promotion of rs3746804 (p. L267P) polymorphism to intracellular SLC52A3a trafficking and riboflavin transportation in esophageal cancer cells
	L. Long; X. X. Pang; F. M. Zeng; X. H. Zhan; Y. H. Xie; F. Pan; W. Wang; L. D. Liao; X. E. Xu; B. Li; L. D. Wang; Z. J. Chang; E. M. Li and L. Y. Xu
	2021
发表期刊	Amino Acids
ISSN	0939-4451
卷号	53 期号:8 页码:1197-1209
摘要	Riboflavin is an essential micronutrient for normal cellular growth and function. Lack of dietary riboflavin is associated with an increased risk for esophageal squamous cell carcinoma (ESCC). Previous studies have identified that the human riboflavin transporter SLC52A3a isoform (encoded by SLC52A3) plays a prominent role in esophageal cancer cell riboflavin transportation. Furthermore, SLC52A3 gene single nucleotide polymorphisms rs3746804 (T>C, L267P) and rs3746803 (C >T, T278M) are associated with ESCC risk. However, whether SLC52A3a (p.L267P) and (p.T278M) act in riboflavin transportation in esophageal cancer cell remains inconclusive. Here, we constructed the full-length SLC52A3a protein fused to green fluorescent protein (GFP-SLC52A3a-WT and mutants L267P, T278M, and L267P/T278M). It was confirmed by immunofluorescence-based confocal microscopy that SLC52A3a-WT, L267P, T278M, and L267P/T278M expressed in cell membrane, as well as in a variety of intracellular punctate structures. The live cell confocal imaging showed that SLC52A3a-L267P and L267P/T278M increased the intracellular trafficking of SLC52A3a in ESCC cells. Fluorescence recovery after photobleaching of GFP-tagged SLC52A3a meant that intracellular trafficking of SLC52A3a-L267P and L267P/T278M was rapid dynamics process, leading to its stronger ability to transport riboflavin. Taken together, the above results indicated that the rs3746804 (p.L267P) polymorphism promoted intracellular trafficking of SLC52A3a and riboflavin transportation in ESCC cells.
DOI	10.1007/s00726-021-03025-4
URL	查看原文
收录类别	SCI
引用统计
文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/65559
专题	中国科学院长春光学精密机械与物理研究所
推荐引用方式 GB/T 7714	L. Long,X. X. Pang,F. M. Zeng,et al. Promotion of rs3746804 (p. L267P) polymorphism to intracellular SLC52A3a trafficking and riboflavin transportation in esophageal cancer cells[J]. Amino Acids,2021,53(8):1197-1209.
APA	L. Long.,X. X. Pang.,F. M. Zeng.,X. H. Zhan.,Y. H. Xie.,...&E. M. Li and L. Y. Xu.(2021).Promotion of rs3746804 (p. L267P) polymorphism to intracellular SLC52A3a trafficking and riboflavin transportation in esophageal cancer cells.Amino Acids,53(8),1197-1209.
MLA	L. Long,et al."Promotion of rs3746804 (p. L267P) polymorphism to intracellular SLC52A3a trafficking and riboflavin transportation in esophageal cancer cells".Amino Acids 53.8(2021):1197-1209.

条目包含的文件		下载所有文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Promotion of rs37468（5038KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览下载