Changchun Institute of Optics,Fine Mechanics and Physics,CAS
Cell-sorting centrifugal microfluidic chip with a flow rectifier | |
J. Ma; Y. Wu; Y. Liu; Y. Ji; M. Yang and H. Zhu | |
2021 | |
发表期刊 | Lab on a Chip |
ISSN | 14730197 |
卷号 | 21期号:11页码:2129-2141 |
摘要 | Centrifugal microfluidic chips offer rapid, highly integrable and simultaneous multi-channel microfluidic control without relying on external pressure pumps and pipelines. Current centrifugal microfluidic chips mainly separate particles of differing density based on the sedimentation method. However, in some biological cells, the volume difference is more notable than the density difference. In particular, cancer cells are generally larger than normal cells. The instability of particle velocity caused by the non-steady flow of the fluid in the centrifugal microfluidic chip leads to low separation purity of particles of different sizes. Thus, we propose herein a centrifugal microfluidic chip with a flow rectifier that transforms the centrifugal non-steady flow into locally steady flow with continuous flow. This chip resolves the problems caused by particle sedimentation in the sample chamber and non-steady flow and greatly improves the recovery ratio and separation purity of target particles. Therefore, it can be used to separate particles of differing size. The experimental results show that the chip can separate an equal-volume mixture of 25 m and 12 m polystyrene particles diluted 50 times with a ratio of 1:6 and obtain a recovery ratio and separation purity better than 95% for the 25 m particles. In addition, rare tumour cells are separated from high-concentration white blood cells (ratio 1:25) with a recovery ratio of 90.4% 2.4% and separation purity of 83.0% 3.8%. In conclusion, this chip is promising for sorting of various biological cells and has significant potential for use in biomedical and clinical applications. The Royal Society of Chemistry 2021. |
DOI | 10.1039/d1lc00217a |
URL | 查看原文 |
收录类别 | SCI ; EI |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.ciomp.ac.cn/handle/181722/65089 |
专题 | 中国科学院长春光学精密机械与物理研究所 |
推荐引用方式 GB/T 7714 | J. Ma,Y. Wu,Y. Liu,et al. Cell-sorting centrifugal microfluidic chip with a flow rectifier[J]. Lab on a Chip,2021,21(11):2129-2141. |
APA | J. Ma,Y. Wu,Y. Liu,Y. Ji,&M. Yang and H. Zhu.(2021).Cell-sorting centrifugal microfluidic chip with a flow rectifier.Lab on a Chip,21(11),2129-2141. |
MLA | J. Ma,et al."Cell-sorting centrifugal microfluidic chip with a flow rectifier".Lab on a Chip 21.11(2021):2129-2141. |
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