Cell-sorting centrifugal microfluidic chip with a flow rectifier

doi:10.1039/d1lc00217a

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	Cell-sorting centrifugal microfluidic chip with a flow rectifier
	J. Ma; Y. Wu; Y. Liu; Y. Ji; M. Yang and H. Zhu
	2021
发表期刊	Lab on a Chip
ISSN	14730197
卷号	21 期号:11 页码:2129-2141
摘要	Centrifugal microfluidic chips offer rapid, highly integrable and simultaneous multi-channel microfluidic control without relying on external pressure pumps and pipelines. Current centrifugal microfluidic chips mainly separate particles of differing density based on the sedimentation method. However, in some biological cells, the volume difference is more notable than the density difference. In particular, cancer cells are generally larger than normal cells. The instability of particle velocity caused by the non-steady flow of the fluid in the centrifugal microfluidic chip leads to low separation purity of particles of different sizes. Thus, we propose herein a centrifugal microfluidic chip with a flow rectifier that transforms the centrifugal non-steady flow into locally steady flow with continuous flow. This chip resolves the problems caused by particle sedimentation in the sample chamber and non-steady flow and greatly improves the recovery ratio and separation purity of target particles. Therefore, it can be used to separate particles of differing size. The experimental results show that the chip can separate an equal-volume mixture of 25 m and 12 m polystyrene particles diluted 50 times with a ratio of 1:6 and obtain a recovery ratio and separation purity better than 95% for the 25 m particles. In addition, rare tumour cells are separated from high-concentration white blood cells (ratio 1:25) with a recovery ratio of 90.4% 2.4% and separation purity of 83.0% 3.8%. In conclusion, this chip is promising for sorting of various biological cells and has significant potential for use in biomedical and clinical applications. The Royal Society of Chemistry 2021.
DOI	10.1039/d1lc00217a
URL	查看原文
收录类别	SCI ; EI
引用统计	被引频次：14[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/65089
专题	中国科学院长春光学精密机械与物理研究所
推荐引用方式 GB/T 7714	J. Ma,Y. Wu,Y. Liu,et al. Cell-sorting centrifugal microfluidic chip with a flow rectifier[J]. Lab on a Chip,2021,21(11):2129-2141.
APA	J. Ma,Y. Wu,Y. Liu,Y. Ji,&M. Yang and H. Zhu.(2021).Cell-sorting centrifugal microfluidic chip with a flow rectifier.Lab on a Chip,21(11),2129-2141.
MLA	J. Ma,et al."Cell-sorting centrifugal microfluidic chip with a flow rectifier".Lab on a Chip 21.11(2021):2129-2141.

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