Single-cell Raman spectrum extraction from clinic biosamples

doi:10.1002/jrs.5984

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	Single-cell Raman spectrum extraction from clinic biosamples
	X. X. Han,Y. H. Wu,M. B. Chi,S. J. Gao and Q. Wang
	2020
发表期刊	Journal of Raman Spectroscopy
ISSN	0377-0486
卷号	51 期号:11 页码:2255-2264
摘要	Raman spectra of clinical samples are often affected by both the substrate and background fluorescence. The overlay of the substrate spectrum, biological Raman spectrum, and the fluorescence spectrum significantly affects the identification of biometrics. In this paper, we propose a specific-scale analysis algorithm and a zero-order Savitzky-Golay filtering algorithm combining local minima to separate the substrate and background fluorescent, respectively. The specific-scale analysis algorithm based on wavelet transform can realize the linear separation of the substrate spectrum through multiresolution analysis. The zero-order Savitzky-Golay filter algorithm combining local minima is an empirical algorithm. It estimates the background fluorescence by building a smooth curve that passes through local minima. We tested our algorithms with simulated spectra and with the Raman spectra of clinical biosamples recorded on glass. The analysis results of three gastric cancer cell lines indicated that the classification error of the processed spectrum decreased significantly.
DOI	10.1002/jrs.5984
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收录类别	SCI
语种	英语
引用统计
文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/64890
专题	中国科学院长春光学精密机械与物理研究所
推荐引用方式 GB/T 7714	X. X. Han,Y. H. Wu,M. B. Chi,S. J. Gao and Q. Wang. Single-cell Raman spectrum extraction from clinic biosamples[J]. Journal of Raman Spectroscopy,2020,51(11):2255-2264.
APA	X. X. Han,Y. H. Wu,M. B. Chi,S. J. Gao and Q. Wang.(2020).Single-cell Raman spectrum extraction from clinic biosamples.Journal of Raman Spectroscopy,51(11),2255-2264.
MLA	X. X. Han,Y. H. Wu,M. B. Chi,S. J. Gao and Q. Wang."Single-cell Raman spectrum extraction from clinic biosamples".Journal of Raman Spectroscopy 51.11(2020):2255-2264.

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