Protein Kinase D3 promotes the cell proliferation by activating the ERK1/c-MYC axis in breast cancer

doi:10.1111/jcmm.14772

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	Protein Kinase D3 promotes the cell proliferation by activating the ERK1/c-MYC axis in breast cancer
	Y. Liu,H. Song,S. Y. Yu,K. H. Huang,X. X. Ma,Y. H. Zhou,S. Yu,J. Z. Zhang and L. M. Chen
	2020
发表期刊	Journal of Cellular and Molecular Medicine
ISSN	1582-1838
卷号	24 期号:3 页码:2135-2144
摘要	Breast cancer is the second leading death cause of cancer death for all women. Previous study suggested that Protein Kinase D3 (PRKD3) was involved in breast cancer progression. In addition, the protein level of PRKD3 in triple-negative breast adenocarcinoma was higher than that in normal breast tissue. However, the oncogenic mechanisms of PRKD3 in breast cancer is not fully investigated. Multi-omic data showed that ERK1/c-MYC axis was identified as a major pivot in PRKD3-mediated downstream pathways. Our study provided the evidence to support that the PRKD3/ERK1/c-MYC pathway play an important role in breast cancer progression. We found that knocking out PRKD3 by performing CRISPR/Cas9 genome engineering technology suppressed phosphorylation of both ERK1 and c-MYC but did not down-regulate ERK1/2 expression or phosphorylation of ERK2. The inhibition of ERK1 and c-MYC phosphorylation further led to the lower protein level of c-MYC and then reduced the expression of the c-MYC target genes in breast cancer cells. We also found that loss of PRKD3 reduced the rate of the cell proliferation in vitro and tumour growth in vivo, whereas ectopic (over)expression of PRKD3, ERK1 or c-MYC in the PRKD3-knockout breast cells reverse the suppression of the cell proliferation and tumour growth. Collectively, our data strongly suggested that PRKD3 likely promote the cell proliferation in the breast cancer cells by activating ERK1-c-MYC axis.
DOI	10.1111/jcmm.14772
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收录类别	SCI
语种	英语
引用统计
文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/64817
专题	中国科学院长春光学精密机械与物理研究所
推荐引用方式 GB/T 7714	Y. Liu,H. Song,S. Y. Yu,K. H. Huang,X. X. Ma,Y. H. Zhou,S. Yu,J. Z. Zhang and L. M. Chen. Protein Kinase D3 promotes the cell proliferation by activating the ERK1/c-MYC axis in breast cancer[J]. Journal of Cellular and Molecular Medicine,2020,24(3):2135-2144.
APA	Y. Liu,H. Song,S. Y. Yu,K. H. Huang,X. X. Ma,Y. H. Zhou,S. Yu,J. Z. Zhang and L. M. Chen.(2020).Protein Kinase D3 promotes the cell proliferation by activating the ERK1/c-MYC axis in breast cancer.Journal of Cellular and Molecular Medicine,24(3),2135-2144.
MLA	Y. Liu,H. Song,S. Y. Yu,K. H. Huang,X. X. Ma,Y. H. Zhou,S. Yu,J. Z. Zhang and L. M. Chen."Protein Kinase D3 promotes the cell proliferation by activating the ERK1/c-MYC axis in breast cancer".Journal of Cellular and Molecular Medicine 24.3(2020):2135-2144.

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