A Pipeline-Based Oil-Bath PCR Method for Bacteria Detection

doi:10.1109/access.2020.2996201

CIOMP OpenIR

	A Pipeline-Based Oil-Bath PCR Method for Bacteria Detection
	D. Wu,B. Shi,B. Li and W. M. Wu
	2020
发表期刊	Ieee Access
ISSN	2169-3536
卷号	8 页码:99598-99604
摘要	Polymerase chain reaction (PCR) technology is an essential technology for point-of-care testing (POCT) application. To realize the simple, portable and fast detection of bacteria has always been an important technology. In this paper, we developed a pipeline-based oil-bath PCR method, to achieve the PCR amplification and detection for bacteria. We assembled a small-sized system with a uniform temperature environment and a stable heating module. By comparing the PCR performance with the other two existing pipeline-based methods on the reactions of both DNA fragment of H7N9 avian influenza and Escherichia Coli, the results show that the pipeline-based oil-bath PCR method is fully capable of the amplification and detection of both DNA fragment and bacteria. At the meanwhile, we performed two sets of tests, the experiment of pipeline diameter change and bacterial concentration gradient, to verify the sensitivity and universality of this method. The results show that the pipeline-based oil-bath PCR method developed by us not only gets rid of the complicated preparations, offers advantages of simple operation, low-cost and user-friendly, but also provides a stable and portable foundation for our later work.
DOI	10.1109/access.2020.2996201
URL	查看原文
收录类别	SCI ; EI
语种	英语
引用统计
文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/64792
专题	中国科学院长春光学精密机械与物理研究所
推荐引用方式 GB/T 7714	D. Wu,B. Shi,B. Li and W. M. Wu. A Pipeline-Based Oil-Bath PCR Method for Bacteria Detection[J]. Ieee Access,2020,8:99598-99604.
APA	D. Wu,B. Shi,B. Li and W. M. Wu.(2020).A Pipeline-Based Oil-Bath PCR Method for Bacteria Detection.Ieee Access,8,99598-99604.
MLA	D. Wu,B. Shi,B. Li and W. M. Wu."A Pipeline-Based Oil-Bath PCR Method for Bacteria Detection".Ieee Access 8(2020):99598-99604.

条目包含的文件		下载所有文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Wu-2020-A Pipeline-B（6874KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览下载