Off-Chip Vertical Step Emulsification Droplets Preparation Device Applied for Droplet Digital PCR

doi:10.1002/admi.202001074

CIOMP OpenIR

	Off-Chip Vertical Step Emulsification Droplets Preparation Device Applied for Droplet Digital PCR
	B. Shi,D. Wu,Y. Y. Jiang,J. R. An and W. M. Wu
	2020
发表期刊	Advanced Materials Interfaces
ISSN	2196-7350
卷号	7 期号:22 页码:7
摘要	High efficient and stable droplets preparation is an essential technology in digital PCR (dPCR) and other analytical fields. In this paper, a microfluidic device called vertical step emulsification (VSE) device to produce emulsion droplets rapidly off-chip is developed. VSE device prepares droplets by perfluoroalkoxy (PFA) capillary and microcentrifuge tube, when the continuous water phase in PFA capillary jets into the static oil phase in the microcentrifuge tube at a certain speed, the continuous water phase is emulsified into stable droplets due to the sudden change of surface tension. In order to control the droplet size accurately, a general screw mechanism to control the gap between the PFA capillary outlet and the bottom of the inner wall of microcentrifuge tube is used. Meanwhile, dPCR tests with high dynamic range throughput droplets from VSE device are performed. The results show that the off-chip droplet preparation device developed by the authors not only gets rid of the complicated control structure, has the advantages of low cost, easy operation, and user-friendly, but also provides a sustainable development scheme for the preparation of high-throughput and stable droplets in a short time.
DOI	10.1002/admi.202001074
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收录类别	SCI ; EI
语种	英语
引用统计
文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/64741
专题	中国科学院长春光学精密机械与物理研究所
推荐引用方式 GB/T 7714	B. Shi,D. Wu,Y. Y. Jiang,J. R. An and W. M. Wu. Off-Chip Vertical Step Emulsification Droplets Preparation Device Applied for Droplet Digital PCR[J]. Advanced Materials Interfaces,2020,7(22):7.
APA	B. Shi,D. Wu,Y. Y. Jiang,J. R. An and W. M. Wu.(2020).Off-Chip Vertical Step Emulsification Droplets Preparation Device Applied for Droplet Digital PCR.Advanced Materials Interfaces,7(22),7.
MLA	B. Shi,D. Wu,Y. Y. Jiang,J. R. An and W. M. Wu."Off-Chip Vertical Step Emulsification Droplets Preparation Device Applied for Droplet Digital PCR".Advanced Materials Interfaces 7.22(2020):7.

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