A novel strategy for creating a new system of third-generation hybrid rice technology using a cytoplasmic sterility gene and a genic male-sterile gene

doi:10.1111/pbi.13457

CIOMP OpenIR

	A novel strategy for creating a new system of third-generation hybrid rice technology using a cytoplasmic sterility gene and a genic male-sterile gene
	S. F. Song,T. K. Wang,Y. X. Li,J. Hu,R. F. Kan,M. D. Qiu,Y. D. Deng,P. X. Liu,L. C. Zhang,H. Dong,C. X. Li,D. Yu,X. Q. Li,D. Y. Yuan,L. P. Yuan and L. Li
	2020
发表期刊	Plant Biotechnology Journal
ISSN	1467-7644
页码	10
摘要	Heterosis utilization is the most effective way to improve rice yields. The cytoplasmic male-sterility (CMS) and photoperiod/thermosensitive genic male-sterility (PTGMS) systems have been widely used in rice production. However, the rate of resource utilization for the CMS system hybrid rice is low, and the hybrid seed production for the PTGMS system is affected by the environment. The technical limitations of these two breeding methods restrict the rapid development of hybrid rice. The advantages of the genic male-sterility (GMS) rice, such as stable sterility and free combination, can fill the gaps of the first two generations of hybrid rice technology. At present, the third-generation hybrid rice breeding technology is being used to realize the application of GMS materials in hybrid rice. This study aimed to use an artificial CMS gene as a pollen killer to create a smart sterile line for hybrid rice production. The clustered regularly interspaced short palindromic repeats/CRISPR-associated 9 (CRISPR/Cas9) technology was used to successfully obtain aCYP703A3-deficient male-sterile mutant containing no genetically modified component in the genetic background of indica 9311. Through young ear callus transformation, this mutant was transformed with three sets of element-linked expression vectors, including pollen fertility restoration geneCYP703A3, pollen-lethality geneorfH79and selection marker geneDsRed2. The maintainer 9311-3B with stable inheritance was obtained, which could realize the batch breeding of GMS materials. Further, the sterile line 9311-3A and restorer lines were used for hybridization, and a batch of superior combinations of hybrid rice was obtained.
DOI	10.1111/pbi.13457
URL	查看原文
收录类别	SCI
语种	英语
引用统计
文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/64734
专题	中国科学院长春光学精密机械与物理研究所
推荐引用方式 GB/T 7714	S. F. Song,T. K. Wang,Y. X. Li,J. Hu,R. F. Kan,M. D. Qiu,Y. D. Deng,P. X. Liu,L. C. Zhang,H. Dong,C. X. Li,D. Yu,X. Q. Li,D. Y. Yuan,L. P. Yuan and L. Li. A novel strategy for creating a new system of third-generation hybrid rice technology using a cytoplasmic sterility gene and a genic male-sterile gene[J]. Plant Biotechnology Journal,2020:10.
APA	S. F. Song,T. K. Wang,Y. X. Li,J. Hu,R. F. Kan,M. D. Qiu,Y. D. Deng,P. X. Liu,L. C. Zhang,H. Dong,C. X. Li,D. Yu,X. Q. Li,D. Y. Yuan,L. P. Yuan and L. Li.(2020).A novel strategy for creating a new system of third-generation hybrid rice technology using a cytoplasmic sterility gene and a genic male-sterile gene.Plant Biotechnology Journal,10.
MLA	S. F. Song,T. K. Wang,Y. X. Li,J. Hu,R. F. Kan,M. D. Qiu,Y. D. Deng,P. X. Liu,L. C. Zhang,H. Dong,C. X. Li,D. Yu,X. Q. Li,D. Y. Yuan,L. P. Yuan and L. Li."A novel strategy for creating a new system of third-generation hybrid rice technology using a cytoplasmic sterility gene and a genic male-sterile gene".Plant Biotechnology Journal (2020):10.

条目包含的文件		下载所有文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Song-A novel strateg（10435KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览下载