Mitochondria-Immobilized Unimolecular Fluorescent Probe for Multiplexing Imaging of Living Cancer Cells

doi:10.1021/acs.analchem.0c01046

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	Mitochondria-Immobilized Unimolecular Fluorescent Probe for Multiplexing Imaging of Living Cancer Cells
	N. S. Zhu,X. L. Guo,S. R. Pang,Y. L. Chang,X. M. Liu,Z. Shi and S. H. Feng
	2020
发表期刊	Analytical Chemistry
ISSN	0003-2700
卷号	92 期号:16 页码:11103-11110
摘要	Cancer incidence and mortality are fast growing worldwide. Recently, multiplexing imaging methods have been reported to be vital for cancer diagnosis and therapy. Fluorescence imaging, which has intrinsic capabilities for multiplexing imaging, is suitable and ripe for cancer imaging. In biomedical research, using a single probe for multiplexing imaging can avoid larger invasive effects and ensure the same spatiotemporal distributions and metabolisms of the probes, which has advantages over using multiple probes. Therefore, developing unimolecular fluorescent probes for multiplexing imaging of living cancer cells is meaningful. We herein report a unimolecular fluorescent probe (ZED) that simultaneously detects cysteine/homocysteine, hypochlorous acid, mitochondrial membrane potential (Delta psi(m)), and opening of the mitochondrial permeability transition (MPT) pore in cells. These four analytes are key indicators predominantly associated with multiple aspects of carcinogenesis and cancer therapy in living cells. Besides, ZED also differentiates MCF-7 cells from HBL-100 cells. The sensing process is fast, selective, and sensitive in living cancer cells. As far as we know, ZED is the first probe that simultaneously detects four analytes in cells and the first probe that simultaneously detects Delta psi(m) and opening of the MPT pore in mitochondria.
DOI	10.1021/acs.analchem.0c01046
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收录类别	SCI ; EI
语种	英语
引用统计
文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/64689
专题	中国科学院长春光学精密机械与物理研究所
推荐引用方式 GB/T 7714	N. S. Zhu,X. L. Guo,S. R. Pang,Y. L. Chang,X. M. Liu,Z. Shi and S. H. Feng. Mitochondria-Immobilized Unimolecular Fluorescent Probe for Multiplexing Imaging of Living Cancer Cells[J]. Analytical Chemistry,2020,92(16):11103-11110.
APA	N. S. Zhu,X. L. Guo,S. R. Pang,Y. L. Chang,X. M. Liu,Z. Shi and S. H. Feng.(2020).Mitochondria-Immobilized Unimolecular Fluorescent Probe for Multiplexing Imaging of Living Cancer Cells.Analytical Chemistry,92(16),11103-11110.
MLA	N. S. Zhu,X. L. Guo,S. R. Pang,Y. L. Chang,X. M. Liu,Z. Shi and S. H. Feng."Mitochondria-Immobilized Unimolecular Fluorescent Probe for Multiplexing Imaging of Living Cancer Cells".Analytical Chemistry 92.16(2020):11103-11110.

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