Low-Cost Battery-Powered and User-Friendly Real-Time Quantitative PCR System for the Detection of Multigene

doi:10.3390/mi11040435

CIOMP OpenIR

	Low-Cost Battery-Powered and User-Friendly Real-Time Quantitative PCR System for the Detection of Multigene
	J. R. An,Y. Y. Jiang,B. Shi,D. Wu and W. M. Wu
	2020
发表期刊	Micromachines
卷号	11 期号:4 页码:9
摘要	Real-time polymerase chain reaction (PCR) is the standard for nucleic acid detection and plays an important role in many fields. A new chip design is proposed in this study to avoid the use of expensive instruments for hydrophobic treatment of the surface, and a new injection method solves the issue of bubbles formed during the temperature cycle. We built a battery-powered real-time PCR device to follow polymerase chain reaction using fluorescence detection and developed an independently designed electromechanical control system and a fluorescence analysis software to control the temperature cycle, the photoelectric detection coupling, and the automatic analysis of the experimental data. The microchips and the temperature cycling system cost USD 100. All the elements of the device are available through open access, and there are no technical barriers. The simple structure and manipulation allows beginners to build instruments and perform PCR tests after only a short tutorial. The device is used for analysis of the amplification curve and the melting curve of multiple target genes to demonstrate that our instrument has the same accuracy and stability as a commercial instrument.
DOI	10.3390/mi11040435
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收录类别	SCI ; EI
语种	英语
引用统计
文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/64660
专题	中国科学院长春光学精密机械与物理研究所
推荐引用方式 GB/T 7714	J. R. An,Y. Y. Jiang,B. Shi,D. Wu and W. M. Wu. Low-Cost Battery-Powered and User-Friendly Real-Time Quantitative PCR System for the Detection of Multigene[J]. Micromachines,2020,11(4):9.
APA	J. R. An,Y. Y. Jiang,B. Shi,D. Wu and W. M. Wu.(2020).Low-Cost Battery-Powered and User-Friendly Real-Time Quantitative PCR System for the Detection of Multigene.Micromachines,11(4),9.
MLA	J. R. An,Y. Y. Jiang,B. Shi,D. Wu and W. M. Wu."Low-Cost Battery-Powered and User-Friendly Real-Time Quantitative PCR System for the Detection of Multigene".Micromachines 11.4(2020):9.

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