Fingerprinting Bacterial Metabolic Response to Erythromycin by Raman-Integrated Mid-Infrared Photothermal Microscopy

doi:10.1021/acs.analchem.0c02489

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	Fingerprinting Bacterial Metabolic Response to Erythromycin by Raman-Integrated Mid-Infrared Photothermal Microscopy
	J. B. Xu,X. J. Li,Z. Y. Guo,W. E. Huang and J. X. Cheng
	2020
发表期刊	Analytical Chemistry
ISSN	0003-2700
卷号	92 期号:21 页码:14459-14465
摘要	We report rapid and sensitive phenotyping of bacterial response to antibiotic treatment at single-cell resolution by a Raman-integrated optical mid-infrared photothermal (MIP) microscope. The MIP microscope successfully detected biochemical changes of bacteria in specific to the acting mechanism of erythromycin with 1 h incubation. Compared to Raman spectroscopy, MIP spectroscopy showed a much larger signal-to-noise ratio at the fingerprint region at an acquisition speed as fast as 1 s per spectrum. The high sensitivity of MIP enabled detection of metabolic changes at antibiotic concentrations below minimum inhibitory concentration (MIC). Meanwhile, the single-cell resolution of the technique allowed observation of heteroresistance within one bacterial population, which is of great clinical relevance. This study showcases characterizing antibiotic response as one of the many possibilities of applying MIP microscopy to single-cell biology.
DOI	10.1021/acs.analchem.0c02489
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收录类别	SCI ; EI
语种	英语
引用统计
文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/64528
专题	中国科学院长春光学精密机械与物理研究所
推荐引用方式 GB/T 7714	J. B. Xu,X. J. Li,Z. Y. Guo,W. E. Huang and J. X. Cheng. Fingerprinting Bacterial Metabolic Response to Erythromycin by Raman-Integrated Mid-Infrared Photothermal Microscopy[J]. Analytical Chemistry,2020,92(21):14459-14465.
APA	J. B. Xu,X. J. Li,Z. Y. Guo,W. E. Huang and J. X. Cheng.(2020).Fingerprinting Bacterial Metabolic Response to Erythromycin by Raman-Integrated Mid-Infrared Photothermal Microscopy.Analytical Chemistry,92(21),14459-14465.
MLA	J. B. Xu,X. J. Li,Z. Y. Guo,W. E. Huang and J. X. Cheng."Fingerprinting Bacterial Metabolic Response to Erythromycin by Raman-Integrated Mid-Infrared Photothermal Microscopy".Analytical Chemistry 92.21(2020):14459-14465.

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