Ascorbic Acid-PEI Carbon Dots with Osteogenic Effects as miR-2861 Carriers to Effectively Enhance Bone Regeneration

doi:10.1021/acsami.0c15425

CIOMP OpenIR

	Ascorbic Acid-PEI Carbon Dots with Osteogenic Effects as miR-2861 Carriers to Effectively Enhance Bone Regeneration
	W. H. Bu, X. W. Xu, Z. L. Wang, N. Q. Jin, L. L. Liu, J. Liu, S. J. Zhu, K. Zhang, R. Jelinek, D. Zhou, H. C. Sun and B. Yang
	2020
发表期刊	Acs Applied Materials & Interfaces
ISSN	1944-8244
卷号	12 期号:45 页码:50287-50302
摘要	Nucleic acid transfer has shown significant potential in the treatment of bone damage because of its long lasting local effect and lower cost. Nonviral vectors, such as nanomaterials, with higher biocompatibility are increasedly applied in the study of bone defect repair. Carbon dots with various reactive groups on the surface not only provide a unique surface to carry therapeutic genes, but also some carbon dots have been reported to promote osteogenic differentiation. The bone regeneration effect of carbon dots in vivo, however, is rarely investigated. MiR-2861 has revealed osteogenic differentiation effects. In the current study, we created ascorbic acid-PEI carbon dots (CD), which were able to carry miR-2861, by the microwave-assisted pyrolysis method. Results demonstrated that CD had excellent fluorescence stability leading to good fluorescence imaging in vitro and in vivo. CD was efficiently internalized into bone marrow stromal cells (BMSCs) through the clathrin-mediated endocytosis pathway and distributed in the mitochondria, endoplasmic reticulum, lysosome, and nucleus. Results from alkaline phosphatase staining, alizarin red staining, and reverse transcription real-time PCR (RT-QPCR) showed that our CD indeed had osteogenic effects in vitro. Flow cytometry data indicated that CD could efficiently deliver miR-2861 into BMSCs in vitro, and CD carrying miR-2861 (CD@miR) had the strongest osteogenic effects. Analyses of hematology, serum biochemistry, and histology showed that CD and CD@miR did not have cytotoxicity and had higher biocompatibility in vivo. Most interestingly, CD and miR-2861 in the CD@miR could act synergistically to promote osteogenic differentiation in vitro and new bone regeneration in vivo remarkably. Our results clearly indicate that the osteogenic CD delivering osteogenic therapeutic gene, miR-2861, can obtain much stronger bone regeneration ability, suggesting that our CD has great potential in future clinical application.
DOI	10.1021/acsami.0c15425
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收录类别	SCI ; EI
语种	英语
引用统计
文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/64320
专题	中国科学院长春光学精密机械与物理研究所
推荐引用方式 GB/T 7714	W. H. Bu, X. W. Xu, Z. L. Wang, N. Q. Jin, L. L. Liu, J. Liu, S. J. Zhu, K. Zhang, R. Jelinek, D. Zhou, H. C. Sun and B. Yang. Ascorbic Acid-PEI Carbon Dots with Osteogenic Effects as miR-2861 Carriers to Effectively Enhance Bone Regeneration[J]. Acs Applied Materials & Interfaces,2020,12(45):50287-50302.
APA	W. H. Bu, X. W. Xu, Z. L. Wang, N. Q. Jin, L. L. Liu, J. Liu, S. J. Zhu, K. Zhang, R. Jelinek, D. Zhou, H. C. Sun and B. Yang.(2020).Ascorbic Acid-PEI Carbon Dots with Osteogenic Effects as miR-2861 Carriers to Effectively Enhance Bone Regeneration.Acs Applied Materials & Interfaces,12(45),50287-50302.
MLA	W. H. Bu, X. W. Xu, Z. L. Wang, N. Q. Jin, L. L. Liu, J. Liu, S. J. Zhu, K. Zhang, R. Jelinek, D. Zhou, H. C. Sun and B. Yang."Ascorbic Acid-PEI Carbon Dots with Osteogenic Effects as miR-2861 Carriers to Effectively Enhance Bone Regeneration".Acs Applied Materials & Interfaces 12.45(2020):50287-50302.

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