Active-modulated, random-illumination, super-resolution optical fluctuation imaging

doi:10.1039/d0nr03255g

CIOMP OpenIR

	Active-modulated, random-illumination, super-resolution optical fluctuation imaging
	B. J. Wang, Z. J. Liu, L. Zhou, Y. Y. Fei, C. L. Yang, L. Mi, Q. Q. Mu and J. Ma
	2020
Source Publication	Nanoscale
ISSN	2040-3364
Volume	12 Issue:32 Pages:16864-16874
Abstract	Super-resolution optical fluctuation imaging (SOFI) provides subdiffraction resolution based on the analysis of temporal stochastic intensity fluctuations. However, conventional SOFI imaging relies on the intrinsic blinking properties of fluorescent markers and suffers from severe artifacts and signal losses owing to the unmatched blinking on-time ratio. Herein, we propose active-modulated, random-illumination, super-resolution optical fluctuation imaging that allows the traditional SOFI to overcome the effect of the intrinsic impertinent blinking characteristic of fluorescent markers. We demonstrate theoretically and experimentally that this method of active-modulated random illumination can generate random illumination patterns with a controllable blinking on-time ratio to match the high-order SOFI reconstruction considerably reducing the generated artifacts and signal losses. High-order, high-quality images can be obtained with increased lateral resolution.
DOI	10.1039/d0nr03255g
URL	查看原文
Indexed By	SCI ; EI
Language	英语
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Document Type	期刊论文
Identifier	http://ir.ciomp.ac.cn/handle/181722/64286
Collection	中国科学院长春光学精密机械与物理研究所
Recommended Citation GB/T 7714	B. J. Wang, Z. J. Liu, L. Zhou, Y. Y. Fei, C. L. Yang, L. Mi, Q. Q. Mu and J. Ma. Active-modulated, random-illumination, super-resolution optical fluctuation imaging[J]. Nanoscale,2020,12(32):16864-16874.
APA	B. J. Wang, Z. J. Liu, L. Zhou, Y. Y. Fei, C. L. Yang, L. Mi, Q. Q. Mu and J. Ma.(2020).Active-modulated, random-illumination, super-resolution optical fluctuation imaging.Nanoscale,12(32),16864-16874.
MLA	B. J. Wang, Z. J. Liu, L. Zhou, Y. Y. Fei, C. L. Yang, L. Mi, Q. Q. Mu and J. Ma."Active-modulated, random-illumination, super-resolution optical fluctuation imaging".Nanoscale 12.32(2020):16864-16874.

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中国科学院长春光学精密机械与物理研究所