Application of automatic feedback photographing by portable smartphone in PCR

doi:10.1016/j.snb.2019.126782

CIOMP OpenIR

	Application of automatic feedback photographing by portable smartphone in PCR
	Y.Y.Jiang; B.Li; W.M.Wu
	2019
发表期刊	Sensors and Actuators B-Chemical
卷号	298 页码:6
摘要	The present PCR instrument is based on the Peltier semiconductor to achieve temperature cycling, but continuous photographing is not allowed and the self-contained timing software was seriously lagging. Therefore, from the perspective of photoelectric control, the conventional PCR machine was combined with a smartphone and PC, and an optical and electrical feedback automatic fluorescence detection system was designed to achieve quantitative real-time PCR. Through the script, camera timing was controlled by comparing the changes on the screen in the adjacent time, and automatic photo feedback is realized. This experiment has proven that this method can greatly improve the accuracy of the time to photograph and clearness of the photos. The smartphone connected to a computer installed on the PCR can be used after debugging. Compared with quantitative real-time PCR, conventional PCR is more cost-effective on the basis of detection requirements.
关键词	Automatic feedback photographing,Smartphones,Conventional PCR,Quantitative real-time PCR,Fluorescence detection,real-time pcr,fluorescent diagnostic system,immunoassay,amplification
DOI	10.1016/j.snb.2019.126782
URL	查看原文
收录类别	SCI ; EI
语种	英语
引用统计
文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/63302
专题	中国科学院长春光学精密机械与物理研究所
推荐引用方式 GB/T 7714	Y.Y.Jiang,B.Li,W.M.Wu. Application of automatic feedback photographing by portable smartphone in PCR[J]. Sensors and Actuators B-Chemical,2019,298:6.
APA	Y.Y.Jiang,B.Li,&W.M.Wu.(2019).Application of automatic feedback photographing by portable smartphone in PCR.Sensors and Actuators B-Chemical,298,6.
MLA	Y.Y.Jiang,et al."Application of automatic feedback photographing by portable smartphone in PCR".Sensors and Actuators B-Chemical 298(2019):6.

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