Fingerprinting a Living Cell by Raman Integrated Mid-Infrared Photothermal Microscopy

doi:10.1021/acs.analchem.9b02286

CIOMP OpenIR

	Fingerprinting a Living Cell by Raman Integrated Mid-Infrared Photothermal Microscopy
	X.J.Li; D.L.Zhang; Y.R.Bai; W.B.Wang; J.Q.Liang; J.X.Cheng
	2019
发表期刊	Analytical Chemistry
ISSN	0003-2700
卷号	91 期号:16 页码:10750-10756
摘要	Vibrational spectroscopic imaging techniques, based on infrared absorption or Raman scattering, allow for noninvasive chemically specific visualization of biological systems. The infrared and Raman modalities with different selection rules provide complementary information. Specifically, infrared microscopy provides strong signals in the fingerprint region, but suffers from low spatial resolution. Raman microscopy provides submicrometer resolution, but requires a long acquisition time. We developed a system that combines the strengths of both techniques by integrating confocal Raman microspectroscopy to the recently developed mid-infrared photothermal microscopy. This hybrid system is capable of fast infrared photothermal imaging of living cells with submicrometer resolution to identify points of interest, followed by a full-spectrum Raman analysis of the identified objects. In addition, a fingerprint photothermal spectrum can be acquired by scanning the wavelengths of the infrared laser. Comprehensive vibrational fingerprint mapping of live cells, demonstrated in adipocytes and single bacteria, promises broad applications of this technology in biology and material science.
关键词	atomic-force microscopy,infrared-spectroscopy,vibrational,spectroscopy,lipid bodies,identification,accumulation,tissues,tool,Chemistry
DOI	10.1021/acs.analchem.9b02286
URL	查看原文
收录类别	SCI ; EI
语种	英语
引用统计
文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/63241
专题	中国科学院长春光学精密机械与物理研究所
推荐引用方式 GB/T 7714	X.J.Li,D.L.Zhang,Y.R.Bai,et al. Fingerprinting a Living Cell by Raman Integrated Mid-Infrared Photothermal Microscopy[J]. Analytical Chemistry,2019,91(16):10750-10756.
APA	X.J.Li,D.L.Zhang,Y.R.Bai,W.B.Wang,J.Q.Liang,&J.X.Cheng.(2019).Fingerprinting a Living Cell by Raman Integrated Mid-Infrared Photothermal Microscopy.Analytical Chemistry,91(16),10750-10756.
MLA	X.J.Li,et al."Fingerprinting a Living Cell by Raman Integrated Mid-Infrared Photothermal Microscopy".Analytical Chemistry 91.16(2019):10750-10756.

条目包含的文件		下载所有文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Fingerprinting a Liv（2657KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览下载