EGFR-specific CAR-T cells trigger cell lysis in EGFR-positive TNBC

doi:10.18632/aging.102510

CIOMP OpenIR

	EGFR-specific CAR-T cells trigger cell lysis in EGFR-positive TNBC
	Y.Liu; Y.H.Zhou; K.H.Huang; Y.Li; X.J.Fang; L.An; F.F.Wang
	2019
发表期刊	Aging-Us
ISSN	1945-4589
卷号	11 期号:23 页码:11054-11072
摘要	Triple-negative breast cancer (TNBC) is an aggressive cancer subtype for which effective therapies are lacking. Epidermal growth factor receptor (EGFR) is overexpressed in various types of TNBC cells, and several EGFR-specific immunotherapies have been used to treat cancer patients. Chimeric antigen receptor engineered T (CAR-T) cells have also been used as cancer therapies. In this study, we generated two types of EGFR-specific CAR-modified T cells using lentiviral vectors with DNA sequences encoding the scFv regions of two anti-EGFR antibodies. The cytotoxic and antitumor effects of these CAR-modified T cells were examined in cytokine release and cytotoxicity assays in vitro and in tumor growth assays in TNBC cell line- and patient-derived xenograft mouse models. Both types of EGFR-specific CAR-T cells were activated by high-EGFR-expressing TNBC cells and specifically triggered TNBC cell lysis in vitro. Additionally, the CAR-T cells inhibited growth of cell-line- and patient-derived xenograft TNBC tumors in mice. These results suggest that EGFR-specific CAR-T cells might be a promising therapeutic strategy in patients with high-EGFR-expressing TNBC.
关键词	triple-negative breast cancer,human epidermal growth factor receptor,chimeric antigen receptor engineered T cells,negative breast-cancer,growth-factor receptor,therapy,amplification,inhibition,expression,challenges,mutations,her2,Cell Biology,Geriatrics & Gerontology
DOI	10.18632/aging.102510
URL	查看原文
收录类别	SCI
语种	英语
引用统计
文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/63170
专题	中国科学院长春光学精密机械与物理研究所
推荐引用方式 GB/T 7714	Y.Liu,Y.H.Zhou,K.H.Huang,et al. EGFR-specific CAR-T cells trigger cell lysis in EGFR-positive TNBC[J]. Aging-Us,2019,11(23):11054-11072.
APA	Y.Liu.,Y.H.Zhou.,K.H.Huang.,Y.Li.,X.J.Fang.,...&F.F.Wang.(2019).EGFR-specific CAR-T cells trigger cell lysis in EGFR-positive TNBC.Aging-Us,11(23),11054-11072.
MLA	Y.Liu,et al."EGFR-specific CAR-T cells trigger cell lysis in EGFR-positive TNBC".Aging-Us 11.23(2019):11054-11072.

条目包含的文件		下载所有文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
EGFR specific CART c（2027KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览下载