Rotundic acid induces Cas3-MCF-7 cell apoptosis through the p53 pathway

doi:10.3892/ol.2018.9616

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	Rotundic acid induces Cas3-MCF-7 cell apoptosis through the p53 pathway
	M.L.Nan; X.Wang; H.J.Li; D.H.Yu; W.Y.Sun; H.M.Xu; Y.F.He
	2019
发表期刊	Oncology Letters
ISSN	1792-1074
卷号	17 期号:1 页码:630-637
摘要	In the present study, the functions and mechanisms of rotundic acid (RA) underlying its induction of apoptosis in caspase-3-transfected MCF-7 human breast cancer cells (Cas3-MCF-7 cells) were investigated. RA induced apoptosis in Cas3-MCF-7 cells more efficiently compared with that in MCF-7 cells transfected with control plasmid. The results from an MTT assay demonstrated that RA effectively inhibited Cas3-MCF-7 cell viability in a dose-dependent manner and induced cell apoptosis via caspase-3 activity within 12 to 48 h. Western blotting and fluorescence-activated cell sorting demonstrated that RA initiated Cas3-MCF-7 cell apoptosis via p53 activation. The silencing of the p53 gene in the Cas3-MCF-7 cell line led to decreased RA-induced Cas3-MCF-7 cell caspase-3 activity and cell apoptosis. Collectively, the results of the present study indicate that caspase-3 serves a critical function in rotundic acid-induced apoptosis, and suggest that caspase-3 deficiency may contribute to the chemotherapy-resistance of breast cancer. Reconstitution of caspase-3 sensitizes MCF-7 breast cancer cells to chemotherapy. RA has the potential for development as a novel drug combined with reconstitution of caspase-3 gene therapy for the treatment of human breast cancer with caspase-3 deficiency.
关键词	rotundic acid,apoptosis,p53,MCF-7 cells,breast-cancer cells,triterpene acids,caspase 3,expression,reconstitution,proteins,protects,trial,Oncology
DOI	10.3892/ol.2018.9616
URL	查看原文
收录类别	SCI
语种	英语
引用统计
文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/63124
专题	中国科学院长春光学精密机械与物理研究所
推荐引用方式 GB/T 7714	M.L.Nan,X.Wang,H.J.Li,et al. Rotundic acid induces Cas3-MCF-7 cell apoptosis through the p53 pathway[J]. Oncology Letters,2019,17(1):630-637.
APA	M.L.Nan.,X.Wang.,H.J.Li.,D.H.Yu.,W.Y.Sun.,...&Y.F.He.(2019).Rotundic acid induces Cas3-MCF-7 cell apoptosis through the p53 pathway.Oncology Letters,17(1),630-637.
MLA	M.L.Nan,et al."Rotundic acid induces Cas3-MCF-7 cell apoptosis through the p53 pathway".Oncology Letters 17.1(2019):630-637.

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