Evaluation method of ensquared energy based on dynamic factors in gene sequencing system

doi:10.1117/1.Oe.58.5.053102

CIOMP OpenIR

	Evaluation method of ensquared energy based on dynamic factors in gene sequencing system
	Z.Y.Sun; W.Y.Li
	2019
发表期刊	Optical Engineering
ISSN	0091-3286
卷号	58 期号:5 页码:6
摘要	In a gene sequencing system, ensquared energy is an important parameter for base-calling software. Nevertheless, the displacement between wafer and camera, which is caused by vibration and position error of the wafer stage while photographing, will decrease the image quality and further influence the ensquared energy. Our study established the relationship model between the dynamic factors and ensquared energy. Several experiments were carried out on the sequencing platform. The results revealed that the ensquared energy has a linear relationship with the location standard deviation of dynamic errors. In the sequencing platform described, if the ensquared energy value required is larger than 65%, the location standard deviation should be controlled below 140 nm. Furthermore, the ensquared energy calculated by a DNA nanoballs image is accordant with the conclusion in the meantime. The performance parameters of the wafer stage and vibration suppression could be drawn up reasonably. (C) 2019 Society of Photo-Optical Instrumentation Engineers (SPIE).
关键词	gene sequencing,fluorescence microscopy,ensquared energy,dynamic,factors,Optics
DOI	10.1117/1.Oe.58.5.053102
收录类别	SCI ; EI
语种	英语
引用统计
文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/63051
专题	中国科学院长春光学精密机械与物理研究所
推荐引用方式 GB/T 7714	Z.Y.Sun,W.Y.Li. Evaluation method of ensquared energy based on dynamic factors in gene sequencing system[J]. Optical Engineering,2019,58(5):6.
APA	Z.Y.Sun,&W.Y.Li.(2019).Evaluation method of ensquared energy based on dynamic factors in gene sequencing system.Optical Engineering,58(5),6.
MLA	Z.Y.Sun,et al."Evaluation method of ensquared energy based on dynamic factors in gene sequencing system".Optical Engineering 58.5(2019):6.

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