A Novel Self-Activated Mechanism for Stable Liquid Transportation Capable of Continuous-Flow and Real-time Microfluidic PCRs

doi:10.3390/mi10060350

CIOMP OpenIR

	A Novel Self-Activated Mechanism for Stable Liquid Transportation Capable of Continuous-Flow and Real-time Microfluidic PCRs
	D.Wu; B.Shi; B.Li; W.M.Wu
	2019
发表期刊	Micromachines
卷号	10 期号:6 页码:14
摘要	The self-activated micropump capable of velocity-stable transport for both single-phased plug and double-phased droplet through long flow distance inside 3D microchannel is one dream of microfluidic scientists. While several types of passive micropumps have been developed based on different actuation mechanisms, until today, it is still one bottleneck to realize such a satisfied self-activated micropump for the stable delivery of both single and double-phased liquid inside long microchannel (e.g., several meters), due to the lack of innovative mechanism in previous methods. To solve this problem, in this article, we propose a new self-activated pumping mechanism. Herein, an end-opened gas-impermeable quartz capillary is utilized for passive transport. Mechanism of this micropump is systemically studied by both the mathematical modeling and the experimental verifications. Based on the flow assays, it totally confirmed a different pumping principle in this paper, as compared with our previous works. The R-2 value of the overall flow rates inside the 3D microchannel is confirmed as high as 0.999, which is much more homogeneous than other passive pumping formats. Finally, this novel micropump is applied to continuous-flow real-time PCRs (both plug-type and microdroplet-type), with the amplification efficiency reaching 91.5% of the commercial PCR cycler instrument.
关键词	self-activated fluidic mechanism,microdroplet,3D microchannel,real-time PCR,house-made setup,polymerase-chain-reaction,sample injection,syringe,pump,micropump,system,permeability,microchannel,microdevice,Science & Technology - Other Topics,Instruments & Instrumentation
DOI	10.3390/mi10060350
收录类别	SCI ; EI
语种	英语
引用统计
文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/62954
专题	中国科学院长春光学精密机械与物理研究所
推荐引用方式 GB/T 7714	D.Wu,B.Shi,B.Li,et al. A Novel Self-Activated Mechanism for Stable Liquid Transportation Capable of Continuous-Flow and Real-time Microfluidic PCRs[J]. Micromachines,2019,10(6):14.
APA	D.Wu,B.Shi,B.Li,&W.M.Wu.(2019).A Novel Self-Activated Mechanism for Stable Liquid Transportation Capable of Continuous-Flow and Real-time Microfluidic PCRs.Micromachines,10(6),14.
MLA	D.Wu,et al."A Novel Self-Activated Mechanism for Stable Liquid Transportation Capable of Continuous-Flow and Real-time Microfluidic PCRs".Micromachines 10.6(2019):14.

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