Bone formation promoted by bone morphogenetic protein-2 plasmid-loaded porous silica nanoparticles with the involvement of autophagy

doi:10.1039/c9nr07017f

CIOMP OpenIR

	Bone formation promoted by bone morphogenetic protein-2 plasmid-loaded porous silica nanoparticles with the involvement of autophagy
	X.W.Xu; M.L.Sun; D.D.Wang; W.H.Bu; Z.L.Wang; Y.Q.Shen
	2019
发表期刊	Nanoscale
ISSN	2040-3364
卷号	11 期号:45 页码:21953-21963
摘要	Gene therapy is one of the most common and effective ways for the regeneration of defective bone tissue, but even highly efficient gene delivery vectors are insufficient. In this study, bone morphogenetic protein-2 plasmid (pBMP-2) was encapsulated by polyethylenimine-modified porous silica nanoparticles (PPSNs), which were synthesized via an ethyl ether emulsion method. Owing to the high specific surface area and high absorption characteristics, low cytotoxicy PPSNs can efficiently load and protect pBMP-2. The resulting PPSN/pBMP-2 can transfect MC3T3-E1 cells effectively to promote osteogenic differentiation and increase calcium deposition in vitro. Interestingly, the mass of calcium deposition nodules decreased dur to the presence of an autophagy inhibitor, demonstrating that PPSNs stimulated the autophagy pathway. Because of their excellent biocompatibility, high transfection efficiency, and ability to stimulate autophagy, the as-prepared PPSN/pBMP-2 could efficiently transfect local cells in a defect area in vivo. Micro-computed tomography and histological images demonstrated that PPSN/pBMP-2 could efficiently promote new bone formation in a 5 mm sized rat calvarial defect model. Taken together, our newly synthesized PPSNs could efficiently carry pBMP-2 and deliver it to the target cells as well as stimulating the autophagy pathway, resulting in significant osteogenic differentiation and bone regeneration.
关键词	gene-therapy,osteoblast differentiation,induce autophagy,delivery,expression,stimulation,osteoclasts,inhibition,vectors,cells,Chemistry,Science & Technology - Other Topics,Materials Science,Physics
DOI	10.1039/c9nr07017f
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收录类别	SCI ; EI
语种	英语
引用统计
文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/62909
专题	中国科学院长春光学精密机械与物理研究所
推荐引用方式 GB/T 7714	X.W.Xu,M.L.Sun,D.D.Wang,et al. Bone formation promoted by bone morphogenetic protein-2 plasmid-loaded porous silica nanoparticles with the involvement of autophagy[J]. Nanoscale,2019,11(45):21953-21963.
APA	X.W.Xu,M.L.Sun,D.D.Wang,W.H.Bu,Z.L.Wang,&Y.Q.Shen.(2019).Bone formation promoted by bone morphogenetic protein-2 plasmid-loaded porous silica nanoparticles with the involvement of autophagy.Nanoscale,11(45),21953-21963.
MLA	X.W.Xu,et al."Bone formation promoted by bone morphogenetic protein-2 plasmid-loaded porous silica nanoparticles with the involvement of autophagy".Nanoscale 11.45(2019):21953-21963.

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