Analysis of aberrations and performance evaluation of adaptive optics in two-photon light-sheet microscopy

doi:10.1016/j.optcom.2018.10.053

CIOMP OpenIR

	Analysis of aberrations and performance evaluation of adaptive optics in two-photon light-sheet microscopy
	C.H.Zhang; W.Q.Sun; Q.Q.Mu; Z.L.Cao; X.Y.Zhang; S.X.Wang; L.Xuan
	2019
发表期刊	Optics Communications
ISSN	0030-4018
卷号	435 页码:46-53
摘要	Two-photon light-sheet microscopy (TP-LSM) system performance is greatly degraded by specimen-induced aberrations in illumination path, which limit the field of view, axial resolution and excitation efficiency of the system. Adaptive optics (AO) is an effective method for attenuating these effects. For the design and evaluation of an AO system, a comprehensive analysis of the effects of aberrations is needed. In this paper, a TP-LSM system is simulated, and new indexes based on integral intensity are introduced for the evaluation of an aberrated light-sheet. Then, the influences of each Zernike mode and random aberrations on the illumination path of the TP-LSM system are investigated with a numerical simulation method. Results show that high-order aberrations have little effect on the axial resolution and excitation efficiency of the system and only low-order components require correction. The random aberrations varied in strength with the depth of the specimens, so the number of corrected Zernike modes is variable. A general formula is generated for the estimation of the number of modes that should be detected and corrected under different aberrations and different numerical aperture of the objective. The results can provide important guidance in the design and evaluation of AO units for TP-LSM systems.
关键词	Adaptive optics,Aberration correction,Two-photon light-sheet,microscopy,specimen-induced aberrations,resolution,Optics
DOI	10.1016/j.optcom.2018.10.053
收录类别	SCI ; EI
语种	英语
引用统计
文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/62822
专题	中国科学院长春光学精密机械与物理研究所
推荐引用方式 GB/T 7714	C.H.Zhang,W.Q.Sun,Q.Q.Mu,et al. Analysis of aberrations and performance evaluation of adaptive optics in two-photon light-sheet microscopy[J]. Optics Communications,2019,435:46-53.
APA	C.H.Zhang.,W.Q.Sun.,Q.Q.Mu.,Z.L.Cao.,X.Y.Zhang.,...&L.Xuan.(2019).Analysis of aberrations and performance evaluation of adaptive optics in two-photon light-sheet microscopy.Optics Communications,435,46-53.
MLA	C.H.Zhang,et al."Analysis of aberrations and performance evaluation of adaptive optics in two-photon light-sheet microscopy".Optics Communications 435(2019):46-53.

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