Changchun Institute of Optics,Fine Mechanics and Physics,CAS
Ultrasensitive label-free optical microfiber coupler biosensor for detection of cardiac troponin I based on interference turning point effect | |
Zhou, W. C.; Li, K. W.; Wei, Y. L.; Hao, P.![]() | |
2018 | |
发表期刊 | Biosensors & Bioelectronics
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ISSN | 0956-5663 |
卷号 | 106页码:99-104 |
摘要 | Sensitive detection of cardiac biomarkers is critical for clinical diagnostics of myocardial infarction (MI) while such detection is quite challenging due to the ultra-low concentration of cardiac biomarkers. In this work, a label-free immunosensor based on optical microfiber coupler (OMC) has been developed for the ultrasensitive detection of cardiac troponin I (cTnI), a selective and highly sensitive biomarker of acute myocardial infarction (AMI). CTnI monoclonal antibodies were immobilized on the surface of the fiber through polyelectrolyte layer using layer-by-layer deposition technique. For refractive index sensing characterization, an ultra-high sensitivity of 91777.9 nm/RIU was achieved when the OMC works around the dispersion turning point, which is the highest experimental demonstration in the field of fiber-optic evanescent biosensors. For biosensing, the immunosensor with good specificity showed a linear wavelength shift in the range of 2-10 fg/mL and an ultra-low detection limit of 2 fg/mL. Such immunosensors have huge application potential for the detection of cardiac biomarkers of myocardial infarction due to simple detection scheme, quick response time, ease of handling and miniaturation. |
关键词 | Optical microfiber coupler Biosensor Dispersion turning point Cardiac troponin I Ultrasensitivity Regeneration acute myocardial-infarction refractive-index increments electrochemical immunosensor immunoassay biomarkers nanoparticles diagnosis complex silicon arrays Biophysics Biotechnology & Applied Microbiology Chemistry Electrochemistry Science & Technology - Other Topics |
DOI | 10.1016/j.bios.2018.01.061 |
收录类别 | SCI ; EI |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.ciomp.ac.cn/handle/181722/61211 |
专题 | 中国科学院长春光学精密机械与物理研究所 |
推荐引用方式 GB/T 7714 | Zhou, W. C.,Li, K. W.,Wei, Y. L.,et al. Ultrasensitive label-free optical microfiber coupler biosensor for detection of cardiac troponin I based on interference turning point effect[J]. Biosensors & Bioelectronics,2018,106:99-104. |
APA | Zhou, W. C..,Li, K. W..,Wei, Y. L..,Hao, P..,Chi, M. B..,...&Wu, Y. H..(2018).Ultrasensitive label-free optical microfiber coupler biosensor for detection of cardiac troponin I based on interference turning point effect.Biosensors & Bioelectronics,106,99-104. |
MLA | Zhou, W. C.,et al."Ultrasensitive label-free optical microfiber coupler biosensor for detection of cardiac troponin I based on interference turning point effect".Biosensors & Bioelectronics 106(2018):99-104. |
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