Ultrasensitive label-free optical microfiber coupler biosensor for detection of cardiac troponin I based on interference turning point effect

doi:10.1016/j.bios.2018.01.061

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	Ultrasensitive label-free optical microfiber coupler biosensor for detection of cardiac troponin I based on interference turning point effect
	Zhou, W. C.; Li, K. W.; Wei, Y. L.; Hao, P.; Chi, M. B.; Liu, Y. S.; Wu, Y. H.
	2018
发表期刊	Biosensors & Bioelectronics
ISSN	0956-5663
卷号	106 页码:99-104
摘要	Sensitive detection of cardiac biomarkers is critical for clinical diagnostics of myocardial infarction (MI) while such detection is quite challenging due to the ultra-low concentration of cardiac biomarkers. In this work, a label-free immunosensor based on optical microfiber coupler (OMC) has been developed for the ultrasensitive detection of cardiac troponin I (cTnI), a selective and highly sensitive biomarker of acute myocardial infarction (AMI). CTnI monoclonal antibodies were immobilized on the surface of the fiber through polyelectrolyte layer using layer-by-layer deposition technique. For refractive index sensing characterization, an ultra-high sensitivity of 91777.9 nm/RIU was achieved when the OMC works around the dispersion turning point, which is the highest experimental demonstration in the field of fiber-optic evanescent biosensors. For biosensing, the immunosensor with good specificity showed a linear wavelength shift in the range of 2-10 fg/mL and an ultra-low detection limit of 2 fg/mL. Such immunosensors have huge application potential for the detection of cardiac biomarkers of myocardial infarction due to simple detection scheme, quick response time, ease of handling and miniaturation.
关键词	Optical microfiber coupler Biosensor Dispersion turning point Cardiac troponin I Ultrasensitivity Regeneration acute myocardial-infarction refractive-index increments electrochemical immunosensor immunoassay biomarkers nanoparticles diagnosis complex silicon arrays Biophysics Biotechnology & Applied Microbiology Chemistry Electrochemistry Science & Technology - Other Topics
DOI	10.1016/j.bios.2018.01.061
收录类别	SCI ; EI
引用统计
文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/61211
专题	中国科学院长春光学精密机械与物理研究所
推荐引用方式 GB/T 7714	Zhou, W. C.,Li, K. W.,Wei, Y. L.,et al. Ultrasensitive label-free optical microfiber coupler biosensor for detection of cardiac troponin I based on interference turning point effect[J]. Biosensors & Bioelectronics,2018,106:99-104.
APA	Zhou, W. C..,Li, K. W..,Wei, Y. L..,Hao, P..,Chi, M. B..,...&Wu, Y. H..(2018).Ultrasensitive label-free optical microfiber coupler biosensor for detection of cardiac troponin I based on interference turning point effect.Biosensors & Bioelectronics,106,99-104.
MLA	Zhou, W. C.,et al."Ultrasensitive label-free optical microfiber coupler biosensor for detection of cardiac troponin I based on interference turning point effect".Biosensors & Bioelectronics 106(2018):99-104.

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