Improvement in focusing accuracy of DNA sequencing microscope with multi-position laser differential confocal autofocus method

doi:10.1364/oe.26.000887

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	Improvement in focusing accuracy of DNA sequencing microscope with multi-position laser differential confocal autofocus method
	Zhang, X.; Zeng, F.; Li, Y.; Qiao, Y. F.
	2018
发表期刊	Optics Express
ISSN	1094-4087
卷号	26 期号:2 页码:895-904
摘要	High focusing accuracy in microscopes could improve the imaging quality to reduce the error rate in DNA sequencing. We propose a new feedback method to improve the focusing condition to a very high accuracy. A reference laser reflected by the sample is detected by two or more sensors around the confocal point. After acquiring the signals from the out-of-focus positions, online data processing is implemented to provide feedbacks for real-time focus-plane locking on the sample surface. This method provides an accuracy better than 1/10 of the objective depth-of-focus. To balance optical aberrations, a specific optical feedback system should be designed, with athermal design considerations to adapt DNA sequencing work to temperature fluctuations. (c) 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
关键词	focal-length measurement biological nanopore radius measurement system Optics
DOI	10.1364/oe.26.000887
收录类别	SCI ; EI
引用统计
文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/61032
专题	中国科学院长春光学精密机械与物理研究所
推荐引用方式 GB/T 7714	Zhang, X.,Zeng, F.,Li, Y.,et al. Improvement in focusing accuracy of DNA sequencing microscope with multi-position laser differential confocal autofocus method[J]. Optics Express,2018,26(2):895-904.
APA	Zhang, X.,Zeng, F.,Li, Y.,&Qiao, Y. F..(2018).Improvement in focusing accuracy of DNA sequencing microscope with multi-position laser differential confocal autofocus method.Optics Express,26(2),895-904.
MLA	Zhang, X.,et al."Improvement in focusing accuracy of DNA sequencing microscope with multi-position laser differential confocal autofocus method".Optics Express 26.2(2018):895-904.

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