Studies of Solid Phase Immunoassay Based on Near Infrared Upconversion Nanoprobe

doi:10.3788/fgxb20183908.1059

CIOMP OpenIR

	Studies of Solid Phase Immunoassay Based on Near Infrared Upconversion Nanoprobe
	Zhou, Jin; Zhang, Mei-Ling; Zhang, Li; Li, Cui-Xia; Zhao, Hui-Ying; Zhang, You-Lin; Xia, An-Dong; Kong, Xiang-Gui
	2018
发表期刊	Faguang Xuebao/Chinese Journal of Luminescence
ISSN	10007032
卷号	39 期号:8 页码:1059-1065
摘要	Uniformed NaYF4:Yb3+,Tm3+ nanoparticles were synthesized through solvent thermal method in chloride. PAA-based ligand replace strategies were carried out to introduce free carboxylic acid groups which allowed further conjugation with NH2-containing human IgG. The silicon wafer was modified with rabbit anti-goat IgG by using protein G as "bridge", which contributed to eliminate structural or conformational changes of IgG. Owing to the specific antibody recognition to the antigenic, a simple, rapid, efficient and sensitive immunoassay method was developed. The experimental results demonstrate that there is a very good linear correlation between the upconversion luminescence intensity and the amount of goat anti-human IgG in the range of 5~400 nmol/L. The detection limit is as low as 1.82 nmol/L. Our developed detection system shows excellent detection specificity and sensitivity, which provides a new detection method and platform with wide linear range and high sensitivity for diagnosis of major clinical diseases in the future. 2018, Science Press. All right reserved.
关键词	Bioassay Antigens Biosensors Chlorine compounds Diagnosis Immunology Infrared devices Luminescence Silicon wafers Synthesis (chemical)
DOI	10.3788/fgxb20183908.1059
收录类别	EI
引用统计
文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/60820
专题	中国科学院长春光学精密机械与物理研究所
推荐引用方式 GB/T 7714	Zhou, Jin,Zhang, Mei-Ling,Zhang, Li,et al. Studies of Solid Phase Immunoassay Based on Near Infrared Upconversion Nanoprobe[J]. Faguang Xuebao/Chinese Journal of Luminescence,2018,39(8):1059-1065.
APA	Zhou, Jin.,Zhang, Mei-Ling.,Zhang, Li.,Li, Cui-Xia.,Zhao, Hui-Ying.,...&Kong, Xiang-Gui.(2018).Studies of Solid Phase Immunoassay Based on Near Infrared Upconversion Nanoprobe.Faguang Xuebao/Chinese Journal of Luminescence,39(8),1059-1065.
MLA	Zhou, Jin,et al."Studies of Solid Phase Immunoassay Based on Near Infrared Upconversion Nanoprobe".Faguang Xuebao/Chinese Journal of Luminescence 39.8(2018):1059-1065.

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