Rotundic acid enhances the impact of radiological toxicity on MCF-7 cells through the ATM p53 pathway

doi:10.3892/ijo.2018.4544

CIOMP OpenIR

	Rotundic acid enhances the impact of radiological toxicity on MCF-7 cells through the ATM p53 pathway
	Wang, Z. F.; Sun, W. Y.; Yu, D. H.; Zhao, Y.; Xu, H. M.; He, Y. F.; Li, H. J.
	2018
发表期刊	International Journal of Oncology
ISSN	1019-6439
卷号	53 期号:5 页码:2269-2277
摘要	Although radiation therapy is a powerful anticancer modality, radiation-induced stress response and gene expression with adaptive resistance may severely compromise the effectiveness of radiation. The function of rotundic acid (RA) on inducing apoptosis in the human breast cancer cell line MCF-7 has been investigated in a previous study. In the present study, the combined effect of chemotherapy and radiotherapy on reducing side effects was examined. The results of an MTT assay revealed that radiation (0.5, 2 and 10 Gy) effectively inhibit MCF-7 cell viability in a dose-dependent manner, consistent with the effects of RA (2, 5 and 12.5 mu M). Interestingly, a lower dose of radiation (1 Gy) combined with RA (5 mu M) exhibited a greater inhibition efficiency compared with a high dose of radiation alone. Flow cytometry revealed that radiation combined with RA induced the apoptosis of MCF-7 cells. Using western blotting, it was demonstrated that radiation induced the expression of ataxia-telangiectasia mutated (ATM) and p53 protein, and that RA enhanced this effect. On examining the potential underlying mechanism, it was revealed that radiation and RA combined induce Bcl-2-associated X protein expression and cell apoptosis in MCF-7 cells. An ATM inhibitor was able to restore the effect of radiation and RA on inducing MCF-7 cell apoptosis. These results suggest that the ATM/p53 pathway directly participates in radiation and RA-induced apoptosis in MCF-7 cells. RA has the potential for development as a novel drug for the treatment of human breast cancer combined with radiation therapy, given that the combined side effects are reduced.
关键词	rotundic acid radiation apoptosis ataxia-telangiectasia mutated tumor protein p53 breast-cancer ionizing-radiation triterpene acids trial radiotherapy expression therapy risk atm trastuzumab Oncology
DOI	10.3892/ijo.2018.4544
收录类别	SCI
引用统计
文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/60798
专题	中国科学院长春光学精密机械与物理研究所
推荐引用方式 GB/T 7714	Wang, Z. F.,Sun, W. Y.,Yu, D. H.,et al. Rotundic acid enhances the impact of radiological toxicity on MCF-7 cells through the ATM p53 pathway[J]. International Journal of Oncology,2018,53(5):2269-2277.
APA	Wang, Z. F..,Sun, W. Y..,Yu, D. H..,Zhao, Y..,Xu, H. M..,...&Li, H. J..(2018).Rotundic acid enhances the impact of radiological toxicity on MCF-7 cells through the ATM p53 pathway.International Journal of Oncology,53(5),2269-2277.
MLA	Wang, Z. F.,et al."Rotundic acid enhances the impact of radiological toxicity on MCF-7 cells through the ATM p53 pathway".International Journal of Oncology 53.5(2018):2269-2277.

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