Changchun Institute of Optics,Fine Mechanics and Physics,CAS
Improving the Imaging Performance of Plasmonic Structured Illumination Microscopy Using MAP Estimation Method | |
Yu, Mu-Xin; Zhou, Wen-Chao; Wu, Yi-Hui | |
2018 | |
发表期刊 | Guangzi Xuebao/Acta Photonica Sinica |
ISSN | 10044213 |
卷号 | 47期号:4 |
摘要 | The classical restoration algorithm can not effectively recover the full frequency domain information of the object, which leads to the serious optical sidelobes. In this paper, the application of metal nano arrays in structured light illumination is studied, and the problem of optical sidelobes in plasmonic structured illumination microscopy is solved using Maximum A Posteriori (MAP) estimation. The research shows that the MAP estimation method can effectively restore the high spatial frequency information, and through the reasonable selection of optimization parameters to achieve the purpose of suppressing optical sidelobes. At the wavelength of 520 nm, 1.3 numerical aperture, the lateral resolution can be obtained at FWHM of 65 nm, which is about 3.6 times of the traditional fluorescence microscope. This technology has potential application application in the field of life science. 2018, Science Press. All right reserved. |
关键词 | Frequency estimation Fluorescence Fluorescence microscopy Frequency domain analysis Image reconstruction Microscopic examination Plasmonics Restoration Surface plasmons |
DOI | 10.3788/gzxb20184704.0422003 |
收录类别 | EI |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.ciomp.ac.cn/handle/181722/60700 |
专题 | 中国科学院长春光学精密机械与物理研究所 |
推荐引用方式 GB/T 7714 | Yu, Mu-Xin,Zhou, Wen-Chao,Wu, Yi-Hui. Improving the Imaging Performance of Plasmonic Structured Illumination Microscopy Using MAP Estimation Method[J]. Guangzi Xuebao/Acta Photonica Sinica,2018,47(4). |
APA | Yu, Mu-Xin,Zhou, Wen-Chao,&Wu, Yi-Hui.(2018).Improving the Imaging Performance of Plasmonic Structured Illumination Microscopy Using MAP Estimation Method.Guangzi Xuebao/Acta Photonica Sinica,47(4). |
MLA | Yu, Mu-Xin,et al."Improving the Imaging Performance of Plasmonic Structured Illumination Microscopy Using MAP Estimation Method".Guangzi Xuebao/Acta Photonica Sinica 47.4(2018). |
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