Passive Micropump for Highly Stable, Long-Termed, and Large Volume of Droplet Generation/Transport Inside 3D Microchannels Capable of Surfactant-Free and Droplet-Based Thermocycled Reverse Transcription-Polymerase Chain Reactions Based on a Single Thermostatic Heater

doi:10.1021/acs.analchem.8b02271

CIOMP OpenIR

	Passive Micropump for Highly Stable, Long-Termed, and Large Volume of Droplet Generation/Transport Inside 3D Microchannels Capable of Surfactant-Free and Droplet-Based Thermocycled Reverse Transcription-Polymerase Chain Reactions Based on a Single Thermostatic Heater
	Li, Yuanming 1; Jiang, Yangyang 1; Wang, Kangning 1; Wu, Wenming 1,2
	2018-10-16
发表期刊	ANALYTICAL CHEMISTRY
ISSN	0003-2700
卷号	90 期号:20 页码:11925-11932
通讯作者	Wu, Wenming(wuwm@ciomp.ac.cn)
摘要	It is still one key challenge for traditional passive micropumps (e.g., surface tension micropump, hydrostatic micropump, enzymatic micropump, degassed-polydimethylsiloxane (PDMS) micropump, etc.) to transport a large volume of two-phased fluid for a long period. Herein we propose a user-friendly and passive approach to realize the microdroplet generation by waiving expensive or complex equipment. The automation principle is systemically studied in this paper. It is affirmed that this micropump can continuously transport over 2000 mu L of two-phased aqueous/oil microdroplets over a 4 m long 3D microchannel for 8 h. In addition, variations in flow rate are little within each hour-period, and the evaporation bubbles can be well suppressed under high temperature (95 degrees C). As a proof of this concept, the novel micropump is applied to droplet-based continuous flow real-time polymerase chain reactions (PCRs), which only require several disposable syringes for oil/aqueous-phase storage, two 34 gauge needles for droplet generation, a Teflon tube for PCR amplification, and a single thermostatic heater for the thermal cycle. The results suggest this droplet generation method is acceptable for a house-made setup of microfluidic PCRs. Besides, the amplification efficiency of the droplet-based microcontinuous flow PCRs here is much higher than the plug-based microcontinuous flow PCRs in our previous work and reaches 91% of the commercial qPCR thermocycler for the target gene of Rubella virus (Ruby). Without expensive microfabrication instruments, this novel method is more accessible to nonprofessionals than previous reports and would extend the droplet-based applications to in-field and real-time analysis.
DOI	10.1021/acs.analchem.8b02271
关键词[WOS]	CONTINUOUS-FLOW PCR ; MICROFLUIDIC CONTROL ; SAMPLE INJECTION ; SYRINGE PUMP ; DEVICE ; CHIP ; PDMS ; MINIATURIZATION ; AMPLIFICATION ; MICRODEVICE
收录类别	SCI
语种	英语
资助项目	Natural Science Foundation of Jilin Province[20180520112JH] ; National Natural Science Foundation of China[61704169] ; CAS Pioneer Hundred Talents Program ; CAS Pioneer Hundred Talents Program ; National Natural Science Foundation of China[61704169] ; Natural Science Foundation of Jilin Province[20180520112JH]
项目资助者	CAS Pioneer Hundred Talents Program ; National Natural Science Foundation of China ; Natural Science Foundation of Jilin Province
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000447816400027
出版者	AMER CHEMICAL SOC
引用统计
文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/60536
专题	中国科学院长春光学精密机械与物理研究所
通讯作者	Wu, Wenming
作者单位	1.Chinese Acad Sci, Changchun Inst Opt Fine Mech & Phys, State Key Lab Appl Opt, Changchun 130033, Jilin, Peoples R China 2.Fudan Univ, State Key Lab ASIC & Syst, Shanghai 200433, Peoples R China
推荐引用方式 GB/T 7714	Li, Yuanming,Jiang, Yangyang,Wang, Kangning,et al. Passive Micropump for Highly Stable, Long-Termed, and Large Volume of Droplet Generation/Transport Inside 3D Microchannels Capable of Surfactant-Free and Droplet-Based Thermocycled Reverse Transcription-Polymerase Chain Reactions Based on a Single Thermostatic Heater[J]. ANALYTICAL CHEMISTRY,2018,90(20):11925-11932.
APA	Li, Yuanming,Jiang, Yangyang,Wang, Kangning,&Wu, Wenming.(2018).Passive Micropump for Highly Stable, Long-Termed, and Large Volume of Droplet Generation/Transport Inside 3D Microchannels Capable of Surfactant-Free and Droplet-Based Thermocycled Reverse Transcription-Polymerase Chain Reactions Based on a Single Thermostatic Heater.ANALYTICAL CHEMISTRY,90(20),11925-11932.
MLA	Li, Yuanming,et al."Passive Micropump for Highly Stable, Long-Termed, and Large Volume of Droplet Generation/Transport Inside 3D Microchannels Capable of Surfactant-Free and Droplet-Based Thermocycled Reverse Transcription-Polymerase Chain Reactions Based on a Single Thermostatic Heater".ANALYTICAL CHEMISTRY 90.20(2018):11925-11932.