Biocompatibility assay of cellular behavior inside a leaf-inspired biomimetic microdevice at the single-cell level

	Biocompatibility assay of cellular behavior inside a leaf-inspired biomimetic microdevice at the single-cell level
	Wu, W. M. and A. Manz
	2017
发表期刊	Rsc Advances
卷号	7 期号:52
摘要	Herein, we introduce a practical and effective manufacturing methodology for a biomimetic microdevice replicated from the Tilia platyphyllos leaf. With this method, artificial microchambers (of controllable dimension and depth) can be easily integrated into leaf-inspired whole-ordered venation patterns. To display the biocompatibility of this microdevice, we applied it to a long-term (seven days) cell culture and monitored the results. Based on a comprehensive biophysical analysis, including covering cellular deformation, cell migration, cytomembrane tension, extracellular communication, protonema formation, microvilli, and the tethers' dynamic of human melanoma cells inside the device at a single-cell resolution, we were able to verify for the first time a leaf-inspired PDMS microdevice as a biocompatible platform for mammal cell culture, showing promise that such a biomimetic device could be further applied for organ-on-a-chip studies and other biomedical research.
收录类别	sci ; ei
语种	英语
文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/59323
专题	中科院长春光机所知识产出
推荐引用方式 GB/T 7714	Wu, W. M. and A. Manz. Biocompatibility assay of cellular behavior inside a leaf-inspired biomimetic microdevice at the single-cell level[J]. Rsc Advances,2017,7(52).
APA	Wu, W. M. and A. Manz.(2017).Biocompatibility assay of cellular behavior inside a leaf-inspired biomimetic microdevice at the single-cell level.Rsc Advances,7(52).
MLA	Wu, W. M. and A. Manz."Biocompatibility assay of cellular behavior inside a leaf-inspired biomimetic microdevice at the single-cell level".Rsc Advances 7.52(2017).

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