One-step in situ solid-substrate-based whole blood immunoassay based on FRET between upconversion and gold nanoparticles

	One-step in situ solid-substrate-based whole blood immunoassay based on FRET between upconversion and gold nanoparticles
	Li, C. X.; J. Zuo; Q. Q. Li; Y. L. Chang; Y. L. Zhang; L. P. Tu; X. M. Liu; B. Xue; H. Y. Zhao; H. Zhang and X. G. Kong
	2017
发表期刊	Biosensors & Bioelectronics
卷号	92
摘要	Despite their general clinical applications, current fluorescence-based immunoassays are confronted with serious challenges, e.g. the advance serum/ plasma separation and the tedious washing process in current heterogeneous approaches, and aggregation of particles, low sensitivity and the narrow linear range in homogeneous approaches. In this paper, these urgent problems were solved in a novel one-step in situ immunoassay of whole blood samples by combining the traditional fluorescence resonance energy transfer (FRET) technology (between upconversion nanoparticles (UCNPs) and gold nanoparticles (GNPs)) and the solid-substrate based immunoassay technology. The low detection limits of goat IgG (gIgG) as 0.042 mu g/mL in buffers, 0.51 mu g/mL in 20-fold diluted whole blood samples and a wide linear range from 0.75 mu g/mL to 60 mu g/mL in blood samples were achieved. To the best of our knowledge, it is the first one-step in situ solid-substrate based immunoassay of whole blood samples with large linear detection range. This development provides a promising platform for a rapid and sensitive immunoassay of various bio-molecules directly in whole blood without tedious separation, washing steps and aggregation problems.
收录类别	sci ; ei
语种	英语
文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/58989
专题	中科院长春光机所知识产出
推荐引用方式 GB/T 7714	Li, C. X.,J. Zuo,Q. Q. Li,et al. One-step in situ solid-substrate-based whole blood immunoassay based on FRET between upconversion and gold nanoparticles[J]. Biosensors & Bioelectronics,2017,92.
APA	Li, C. X..,J. Zuo.,Q. Q. Li.,Y. L. Chang.,Y. L. Zhang.,...&H. Zhang and X. G. Kong.(2017).One-step in situ solid-substrate-based whole blood immunoassay based on FRET between upconversion and gold nanoparticles.Biosensors & Bioelectronics,92.
MLA	Li, C. X.,et al."One-step in situ solid-substrate-based whole blood immunoassay based on FRET between upconversion and gold nanoparticles".Biosensors & Bioelectronics 92(2017).

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