Resveratrol inhibits the IL-1 beta-induced expression of MMP-13 and IL-6 in human articular chondrocytes via TLR4/MyD88-dependent and-independent signaling cascades

	Resveratrol inhibits the IL-1 beta-induced expression of MMP-13 and IL-6 in human articular chondrocytes via TLR4/MyD88-dependent and-independent signaling cascades
	Gu, H. L.; Y. L. Jiao; X. L. Yu; X. Y. Li; W. Wang; L. F. Ding and L. Liu
	2017
发表期刊	International Journal of Molecular Medicine
卷号	39 期号:3
摘要	The natural polyphenolic compound, resveratrol, has been shown to exhibit anti-osteoarthritic activity. Therefore it is hypothesized that resveratrol may serve as a nutritional supplement to counteract osteoarthritis (OA). However, the mechanisms responsible for these anti-osteoarthritic effects have not yet been fully elucidated. The aim of this study was to determine whether the biological effects of resveratrol against interleukin (IL)-1 beta-induced inflammation in human articular chondrocytes involved both Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (MyD88)-dependent and-independent signaling pathways. Human articular chondrocytes derived from patients with OA were stimulated with IL-1 beta, and then co-treated with resveratrol. Cell viability was subsequently evaluated by MTS assays, and the concentrations of matrix metalloproteinase (MMP)-13 and the pro-inflammatory factor, IL-6, were detected in culture supernatants using ELISA. The mRNA and protein levels of downstream mediators of TLR4/MyD88-dependent and-independent signaling pathways were also assayed by RT-qPCR and western blot analysis, respectively. Our results revealed that resveratrol prevented the IL-1 beta-induced reduction in cell viability. Furthermore, stimulation of the chondrocytes with IL-1 beta resulted in a significant upregulation of TLR4 and downstream targets of both TLR4/MyD88-dependent and-independent signaling pathways that are associated with the synthesis of MMP-13 and IL-6. Correspondingly, IL-1 beta induced catabolic and inflammatory responses were effectively reversed by resveratrol. Taken together, these data suggest that resveratrol exerted protective effects against matrix degradation and inflammation in OA-affected chondrocytes by inhibiting both TLR4/MyD88-dependent and-independent signaling pathways. Thus, resveratrol represents a potential treatment for OA and warrants further investigation.
收录类别	sci ; ei
语种	英语
文献类型	期刊论文
条目标识符	http://ir.ciomp.ac.cn/handle/181722/58912
专题	中科院长春光机所知识产出
推荐引用方式 GB/T 7714	Gu, H. L.,Y. L. Jiao,X. L. Yu,et al. Resveratrol inhibits the IL-1 beta-induced expression of MMP-13 and IL-6 in human articular chondrocytes via TLR4/MyD88-dependent and-independent signaling cascades[J]. International Journal of Molecular Medicine,2017,39(3).
APA	Gu, H. L.,Y. L. Jiao,X. L. Yu,X. Y. Li,W. Wang,&L. F. Ding and L. Liu.(2017).Resveratrol inhibits the IL-1 beta-induced expression of MMP-13 and IL-6 in human articular chondrocytes via TLR4/MyD88-dependent and-independent signaling cascades.International Journal of Molecular Medicine,39(3).
MLA	Gu, H. L.,et al."Resveratrol inhibits the IL-1 beta-induced expression of MMP-13 and IL-6 in human articular chondrocytes via TLR4/MyD88-dependent and-independent signaling cascades".International Journal of Molecular Medicine 39.3(2017).

条目包含的文件		下载所有文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Resveratrol inhibits（688KB）	期刊论文	作者接受稿	开放获取	CC BY-NC-SA	浏览下载